GENOME SIZE AND RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM ANALYSIS OF VIBRIO-CHOLERAE STRAINS BELONGING TO DIFFERENT SEROVARS AND BIOTYPES

被引：13

作者：

CHOUDHURY, SR ^{[1
]}

BHADRA, RK ^{[1
]}

DAS, J ^{[1
]}

机构：

[1] INDIAN INST CHEM BIOL,DIV BIOPHYS,CALCUTTA 700032,W BENGAL,INDIA

来源：

FEMS MICROBIOLOGY LETTERS | 1994年 / 115卷 / 2-3期

关键词：

VIBRIO CHOLERAE; GENOME SIZE; BIOTYPE; SEROTYPE DIFFERENTIATION; PULSED FIELD GEL ELECTROPHORESIS; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

D O I：

10.1016/0378-1097(94)90035-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The genome size of Vibrio cholerae has been determined by pulsed field gel electrophoresis following digestion of chromosomal DNA with endonucleases. The genome size of all the classical strains examined was about 3000 kb and that of El Tor biotype was 2500 kb. The NotI and SfiI digestion patterns of the genomes of several V. cholerae strains belonging to different serovars and biotypes showed distinct restriction fragment length polymorphism (RFLP). RFLP analysis together with the genome size can be used to differentiate strains of different serovars and biotypes of V. cholerae.

引用

页码：329 / 334

页数：6

共 15 条

[1] BETLEY MJ, 1986, ANNU REV MICROBIOL, V40, P577, DOI 10.1146/annurev.mi.40.100186.003045
[2] STRUCTURAL ORGANIZATION OF CHOLERA-TOXIN GENE AND ITS EXPRESSION IN AN ENVIRONMENTAL NONPATHOGENIC STRAIN OF VIBRIO-CHOLERAE
BHATTACHARYYA, U
GHOSH, A
GHOSH, RK
[J]. JOURNAL OF BIOSCIENCES, 1987, 11 (1-4) : 231 - 238
[3] GENOME ORGANIZATION OF THE ANAEROBIC PATHOGEN CLOSTRIDIUM-PERFRINGENS
CANARD, B
COLE, ST
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (17) : 6676 - 6680
[4] CELL-SURFACE CHARACTERISTICS OF ENVIRONMENTAL AND CLINICAL ISOLATES OF VIBRIO-CHOLERAE NON-O1
CHAUDHURI, K
BHADRA, RK
DAS, J
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (11) : 3567 - 3573
[5] DASGUPTA U, 1993, IN PRESS VACCINE
[6] PHYSICAL MAP OF THE CHROMOSOME OF NEISSERIA-GONORRHOEAE FA1090 WITH LOCATIONS OF GENETIC-MARKERS, INCLUDING OPA AND PIL GENES
DEMPSEY, JAF
LITAKER, W
MADHURE, A
SNODGRASS, TL
CANNON, JG
[J]. JOURNAL OF BACTERIOLOGY, 1991, 173 (17) : 5476 - 5486
[7] GENETICS OF VIBRIO-CHOLERAE AND ITS BACTERIOPHAGES
GUIDOLIN, A
MANNING, PA
[J]. MICROBIOLOGICAL REVIEWS, 1987, 51 (02) : 285 - 298
[8] JANDA J M, 1988, Clinical Microbiology Reviews, V1, P245
[9] MANIATIS T, 1982, MOL CLONING LABORATO, P383
[10] DUPLICATION AND AMPLIFICATION OF TOXIN GENES IN VIBRIO-CHOLERAE
MEKALANOS, JJ
[J]. CELL, 1983, 35 (01) : 253 - 263

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