A SYNTHETIC PEPTIDE MIMICS TROPONIN-I FUNCTION IN THE CALCIUM-DEPENDENT REGULATION OF MUSCLE-CONTRACTION

被引:30
作者
VANEYK, JE
STRAUSS, JD
HODGES, RS
RUEGG, JC
机构
[1] UNIV HEIDELBERG,INST PHYSIOL 2,W-6900 HEIDELBERG,GERMANY
[2] UNIV ALBERTA,MRC,PROT STRUCT & FUNCT GRP,EDMONTON T6G 2E1,ALBERTA,CANADA
[3] UNIV ALBERTA,DEPT BIOCHEM,EDMONTON T6G 2E1,ALBERTA,CANADA
关键词
TROPONIN-I; SYNTHETIC PEPTIDE; MUSCLE REGULATION; PROTEIN PROTEIN INTERACTION;
D O I
10.1016/0014-5793(93)81344-Y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A new technique for treating skinned cardiac muscle fibers has been developed in which troponin I is extracted, giving rise to unregulated fibers. The effect of the 12-residue troponin I peptide on these fibers indicates that this region of troponin I is solely responsible for muscle relaxation (inhibition of force). Furthermore, troponin I peptide-troponin C reconstituted fibers are stable through several contraction-relaxation cycles indicating the peptide can switch binding sites between actin and troponin C. The troponin I peptide can substitute for the native protein as part of the calcium-sensitive molecular switch that controls muscle regulation.
引用
收藏
页码:223 / 228
页数:6
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