FERTILE SOMATIC HYBRIDS BETWEEN BRASSICA-NAPUS AND ARABIDOPSIS-THALIANA

被引:36
作者
FORSBERG, J
LANDGREN, M
GLIMELIUS, K
机构
[1] Department of Plant Breeding, Uppsala Genetic Center, Swedish University of Agricultural Sciences, S-75007 Uppsala
关键词
BRASSICA NAPUS; ARABIDOPSIS THALIANA; BRASSICACEAE; SYMMETRICAL SOMATIC HYBRIDS; FERTILITY;
D O I
10.1016/0168-9452(94)90095-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fertile somatic hybrids between Brassica napus and Arabidopsis thaliana were produced by protoplast fusion and enriched by flow sorting. Isoenzyme analysis and hybridization of repetitive DNA sequences from the two parental species to total DNA from the hybrids were used to verify hybrid characteristics of the nuclear genomes. Of 29 shoots, each from a different callus, 25 had a hybrid pattern for at least one of the markers investigated. Analysis of chromosome number revealed that 11 out of 18 hybrids had the sum of the two parental chromosome numbers. Organelle analysis of 18 hybrids showed that ten hybrids had mitochondrial (mt) restriction patterns identical to those of B. napus, while eight displayed a rearranged mtDNA restriction fragment pattern. B. napus chloroplasts (cp) were favoured in the hybrids, and out of 21 analysed hybrids only one plant had A. thaliana cp. Most of the hybrids had intermediate morphological features compared with those of the parental species and 14 out of the 19 tested hybrids were female fertile and set seed after backcrossing to B. napus. Of the female-fertile hybrids, two were also self-fertile.
引用
收藏
页码:213 / 223
页数:11
相关论文
共 53 条
[1]  
ALSHEHBAZ IA, 1984, J ARNOLD ARBORETUM, V65, P343
[2]  
Arumuganathan K., 1991, PLANT MOL BIOL REP, V9, P208, DOI [DOI 10.1007/BF02672069, 10.1007/BF02672069]
[3]   COMPARISON OF ISOLATION AND CULTURE OF MESOPHYLL PROTOPLASTS FROM SEVERAL NICOTIANA SPECIES AND THEIR HYBRIDS [J].
BANKS, MS ;
EVANS, PK .
PLANT SCIENCE LETTERS, 1976, 7 (06) :409-416
[4]   PRODUCTION AND CHARACTERIZATION OF ASYMMETRIC SOMATIC HYBRIDS BETWEEN ARABIDOPSIS-THALIANA AND BRASSICA-NAPUS [J].
BAUERWESTON, B ;
KELLER, W ;
WEBB, J ;
GLEDDIE, S .
THEORETICAL AND APPLIED GENETICS, 1993, 86 (2-3) :150-158
[5]   NUCLEAR-DNA AMOUNTS IN ANGIOSPERMS [J].
BENNETT, MD ;
SMITH, JB .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1976, 274 (933) :227-274
[6]  
Berger B, 1965, ARABIDOPSIS RES, P19
[7]   GENETICS OF ACTIN-RELATED SEQUENCES IN TOMATO [J].
BERNATZKY, R ;
TANKSLEY, SD .
THEORETICAL AND APPLIED GENETICS, 1986, 72 (03) :314-321
[8]   NUCLEOTIDE-SEQUENCE OF THE WHEAT MITOCHONDRIAL GENE FOR SUBUNIT-I OF CYTOCHROME-OXIDASE [J].
BONEN, L ;
BOER, PH ;
MCINTOSH, JE ;
GRAY, MW .
NUCLEIC ACIDS RESEARCH, 1987, 15 (16) :6734-6734
[9]   NUCLEOTIDE-SEQUENCE OF THE F1-ATPASE-ALPHA SUBUNIT GENE FROM MAIZE MITOCHONDRIA [J].
BRAUN, CJ ;
LEVINGS, CS .
PLANT PHYSIOLOGY, 1985, 79 (02) :571-577
[10]   NUCLEAR-MITOCHONDRIAL INTERRELATION IN ANGIOSPERMS [J].
BREIMAN, A ;
GALUN, E .
PLANT SCIENCE, 1990, 71 (01) :3-19