SEPARATION OF ENANTIOMERS OF BENZODIAZEPINES ON THE CHIRAL-AGP COLUMN

The resolution of twenty-five 3-chiral and 5-chiral 1,4-benzodiazepines and related compounds was studied on a Chiral-AGP column. Relationship between the structure and enantioselective retention is discussed stressing the role of hydrophobic and hydrogen-bonding interactions as well as the importance of the conformation of the enantiomers. The majority of the benzodiazepines were separated with high separation factors and high resolution. The enantioselectivity was influenced by the nature and the concentration of the organic modifier in the mobile phase, as well as by the pH. Chiral chromatographic separation was compared with stereoselective binding on native AGP.