TEMPERATURE-SENSITIVE HVJ (SENDAI VIRUS) WITH ALTERED P-POLYPEPTIDE DERIVED FROM PERSISTENTLY INFECTED CELL-LINES

被引:8
作者
OGURA, H
SATO, H
HATANO, M
机构
关键词
D O I
10.1099/0022-1317-55-2-469
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
HVJ [hemagglutinating virus of Japan] isolated from culture fluids of G2 [human giant tumor], THEL [golden hamster embryonic lung] and GM2 [golden hamster fibrosarcoma] cells persistently infected with HVJ (G2-HVJ, THEL-HVJ and GM2-HVJ) were characterized in comparison with wild-type HVJ (HVJo). Viral structural proteins were analyzed by 10% SDS[sodium dodecyl sulfate]-polyacrylamide gel electrophoresis. Only the P polypeptides of all the HVJ clones isolated from G2-HVJ cells had a smaller size MW of 77,000 (77K) than that of HVJo with a MW of 79K. One of 6 clones from THEL-HVJ cells and 1 of 10 clones from GM2-HVJ cells exhibited the same migration pattern of P polypeptide as that of the clones from G2-HVJ cells. The other structural proteins were not different from those of the wild-type virions. All the clones from these carrier cultures were temperature-sensitive and were blocked in early step(s) required for RNA sunthesis. Some mutations(s) associated with P polypeptide could occur during the course of HVJ persistent infection in cell cultures.
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页码:469 / 473
页数:5
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