ENHANCEMENT OF HOST-CELL REACTIVATION OF ULTRAVIOLET-IRRADIATED HERPES-SIMPLEX VIRUS BY CAFFEINE, HYDROXYUREA AND 5-BROMODEOXYURIDINE

被引:10
作者
FOGEL, M
YAMANISHI, K
RAPP, F
机构
[1] PENN STATE UNIV,MILTON S HERSHEY MED CTR,DEPT MICROBIOL,HERSHEY,PA 17033
[2] PENN STATE UNIV,MILTON S HERSHEY MED CTR,SPECIALIZED CANC RES CTR,HERSHEY,PA 17033
关键词
D O I
10.1002/ijc.2910230511
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Enhancement of host cell reactivation (HCR) of ultraviolet (UV)‐irradiated herpes simplex virus (HSV) was demonstrated in cell cultures pretreated with caffeine, hydroxyurea, or 5‐bromodeoxyuridine (Brd Urd). The effect of caffeine on HCR was shown to depend on the time of drug treatment with respect to infection. In cultures treated with caffeine during the course of virus replication, the infectivity of irradiated HSV was reduced about nine‐fold, while cultures pretreated with the drug before infection showed an increase in infectivity. The extent of HCR enhancement depended on the time interval between treatment with caffeine and infection, drug concentration, and the UV irradiation dose to which HSV was exposed. Magnitude of enhancement of HCR by caffeine differed in various cell species. The results suggest that enhanced HCR of UV‐irradiated HSV by DNA antimetabolites is associated with DNA repair activated in consequence of cell DNA damage. Copyright © 1979 Wiley‐Liss, Inc., A Wiley Company
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页码:657 / 662
页数:6
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