TRITIUM LABELING OF SMALL AMOUNTS OF PROTEIN FOR ANALYSIS BY ELECTROPHORESIS ON SODIUM DODECYL SULFATE-POLYACRYLAMIDE SLAB GELS

被引：59

作者：

KUMARASAMY, R ^{[1
]}

SYMONS, RH ^{[1
]}

机构：

[1] UNIV ADELAIDE, DEPT BIOCHEM, ADELAIDE 5001, S AUSTRALIA, AUSTRALIA

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90739-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and reproducible method for the tritium labeling of small amounts of proteins prior to analysis under denaturing conditions on polyacrylamide slab gels is described. The method involves the in vitro labeling of proteins by reductive methylation using formaldehyde and high specific activity [3H]potassium borohydride. Labeled proteins were detected by fluorography after fractionation on polyacrylamide slab gels in the presence of sodium dodecylsulfate. The overall procedure allows the analysis and molecular weight estimation of submicrogram quantities of protein. © 1979.

引用

页码：359 / 363

页数：5

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