INSERTIONAL DISRUPTION OF THE NUSB (SSYB) GENE LEADS TO COLD-SENSITIVE GROWTH OF ESCHERICHIA-COLI AND SUPPRESSION OF THE SECY24 MUTATION

被引:48
作者
TAURA, T
UEGUCHI, C
SHIBA, K
ITO, K
机构
[1] KYOTO UNIV,INST VIRUS RES,KYOTO 60601,JAPAN
[2] JAPANESE FDN CANC RES,INST CANC,TOKYO 170,JAPAN
来源
MOLECULAR & GENERAL GENETICS | 1992年 / 234卷 / 03期
关键词
PROTEIN SECRETION; SUPPRESSOR OF SECY; RIBOFLAVIN BIOSYNTHESIS; NUSB GENE; SSYB MUTATION;
D O I
10.1007/BF00538702
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Escherichia coli gene ssyB was cloned and sequenced. The ssyB63 (Cs) mutation is an insertion mutation in nusB, while the nusB5 (Cs) mutation suppresses sec Y24, indicating that inactivation of nusB causes cold-sensitive cell growth as well as phenotypic suppression of sec Y24. The correct map position of nusB is 9.5 min rather than 11 min as previously assigned. It is located at the distal end of an operon that contains a gene showing significant homology with a Bacillus subtilis gene involved in riboflavin biosynthesis.
引用
收藏
页码:429 / 432
页数:4
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