A COLUMN-SWITCHING TECHNIQUE FOR THE SCREENING OF DIURETICS IN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：17

作者：

SAARINEN, M

SIREN, H

RIEKKOLA, ML

机构：

[1] University of Helsinki, Department of Chemistry, Analytical Chemistry Division, Helsinki, P.O. Box 6

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1993年 / 16卷 / 18期

关键词：

D O I：

10.1080/10826079308019687

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method using column switching and reversed-phase high performance liquid chromatography with diode array detection was developed for the screening of 16 diuretics, probenecid and caffeine in urine. First, a 40-mul sample is injected into a short ODS or ODP extraction column with water as eluent and polar matrix components are eluted to waste. The retained compounds are then eluted in backflush mode into the analytical column by means of a column switching valve and a gradient of 0.05 M phosphate buffer (pH 3.1) and acetonitrile. Wavelengths 230, 270 and 360 nm are used for the detection. Recoveries for spiked urine samples were near 100% and relative retention times showed good repeatability. Detection limits were 0.5-2.0 mug/ml.

引用

页码：4063 / 4078

页数：16

共 13 条

[1]

AHNOFF M, 1985, J CHROMATOGR, V5340, P73

[2] SOLID-PHASE EXTRACTION TECHNIQUES FOR ASSAY OF DIURETICS IN HUMAN URINE SAMPLES [J].