EXPLORING DNA MINOR-GROOVE INTERACTIONS THROUGH A PROBE CONJUGATE IN MAJOR GROOVE - FLUORESCENCE STUDIES ON NETROSIN COMPLEXATION WITH DU-5-AMINODANSYL-DNA

被引：8

作者：

BARAWKAR, DA ^{[1
]}

GANESH, KN ^{[1
]}

机构：

[1] NATL CHEM LAB,DIV ORGAN CHEM,POONA 411008,MAHARASHTRA,INDIA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 203卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.2147

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The binding of netropsin to 5-Aminodansyl-dU-DNA (2-5) in minor groove is shown to correlate with fluorescence changes of dansyl fluorophore located in major groove. This enables a study of a minor groove binding event through crosstalk with conjugated probe in major groove. The dielectric constant of major groove estimated from fluorescence properties of (2-6) is about 55D in contrast to the literature reported minor groove dielectric constant of 20D in the poly[d(AT)]-poly[d(AT)] duplex. (C) 1994 Academic Press, Inc.

引用

页码：53 / 58

页数：6

共 18 条

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