INTERLEUKIN-10 INHIBITS ANTIGEN-INDUCED CELLULAR RECRUITMENT INTO THE AIRWAYS OF SENSITIZED MICE

被引：220

作者：

ZUANYAMORIM, C

HAILE, S

LEDUC, D

DUMAREY, C

HUERRE, M

VARGAFTIG, BB

PRETOLANI, M

机构：

[1] INST PASTEUR,INSERM,UA 285,UNITE PHARMACOL CELLULAIRE,F-75015 PARIS,FRANCE

[2] INST PASTEUR,UNITE HISTOPATHOL,F-75015 PARIS,FRANCE

来源：

JOURNAL OF CLINICAL INVESTIGATION | 1995年 / 95卷 / 06期

关键词：

BRONCHIAL INFLAMMATION; EOSINOPHILS; NEUTROPHILS; TUMOR NECROSIS FACTOR;

D O I：

10.1172/JCI117966

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

This report examines the effect of recombinant murine (rm)IL-10 on antigen-induced cellular recruitment into the airways of sensitized Balb/c mice, The intranasal instillation of 10 mu g ovalbumin induced an early (6-24 h) increase in the number of neutrophils, and a late rise (24-96 h) in that of eosinophils in the bronchoalveolar lavage (BAL) fluid and bronchial tissue. A single intranasal instillation of 0.01-0.1 mu g Of rmIL-10, administered concurrently with ovalbumin, but not 1 or 3 h thereafter, dose-dependently inhibited both airway neutrophilia and eosinophilia. This phenomenon was suppressed by treating the sensitized mice with 1 mg/mouse of a neutralizing anti-IL-10 mAb, which increased significantly ovalbumin-induced neutrophil and eosinophil accumulation in the BAL fluid, These results suggest that antigen stimulation may trigger the in vivo generation of IL-10, which, in turn, participates in the leukocyte infiltration into the airways. rmIL-10 also reduced TNF-alpha release in the BAL fluid observed 1 and 3 h after antigen challenge, Furthermore, the intranasal instillation of an anti-TNF-alpha antiserum to sensitized mice markedly reduced ovalbumin-induced neutrophil and eosinophil accumulation in the BAL fluid. These findings indicate that leukocyte infiltration into the airways of antigen-challenged mice is regulated by IL-10, Furthermore, inhibition of TNF-alpha production by rmIL-10 suggests that allergic airway inflammation and TNF-alpha formation are parallel events in this model.

引用

页码：2644 / 2651

页数：8

共 49 条

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