ON RAPID LABORATORY DIALYSIS

Attempts to devise a practical, rapid, and highly efficient laboratory dialyzer have resulted in the construction of an improved countercurrent “thin film” dialysis column which will clear the tritium count in tritiated water, on a single pass requiring 6-8 min, to the extent of at least six orders of magnitude. Bacitracin, mol wt 1422, can be cleared to the extent of 99+%. Data are presented to show that the column in its present form is useful for desalting peptide, protein, and nucleotide solutions, for proton exchange studies, for various binding studies, and certain bio-assays. It can be used as an ultrafiltration device by using a polymer to provide a higher osmotic pressure in the diffusate than is in the retentate stream. © 1969, American Chemical Society. All rights reserved.