A RAPID ASSAY AND PARTIAL PURIFICATION OF DOPA DECARBOXYLASE

被引：31

作者：

LADURON, P

BELPAIRE, F

机构：

[1] J. F. and C. Heymans Institute of Pharmacology, Ghent University School of Medicine, Ghent

来源：

ANALYTICAL BIOCHEMISTRY | 1968年 / 26卷 / 02期

关键词：

D O I：

10.1016/0003-2697(68)90332-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An assay procedure for dopa decarboxylase has been described. The method uses dopa-C14 as substrate and a butanol extraction under neutral conditions of pH to separate the formed dopamine from dopa. This methods allows a very rapid and particularly simple determination of dopa decarboxylase activity in samples containing low amount of enzyme, such as adrenergic tissues. Dopa decarboxylase and dopamine-β-hydroxylase can be separated and partially purified on the basis of their different sedimentation through a sucrose gradient. © 1968.

引用

页码：210 / &

共 12 条

[1]

ANTON AH, 1962, J PHARMACOL EXP THER, V138, P360

[2] TISSUE FRACTIONATION AND CATECHOLAMINES .I. LATENCY AND ACTIVATION PROPERTIES OF DOPAMINE-BETA-HYDROXYLASE IN ADRENAL MEDULLA [J].