VASOACTIVE-INTESTINAL-PEPTIDE STIMULATES CILIARY MOTILITY IN RABBIT TRACHEAL EPITHELIUM - MODULATION BY NEUTRAL ENDOPEPTIDASE

被引：23

作者：

SAKAI, N ^{[1
]}

TAMAOKI, J ^{[1
]}

KOBAYASHI, K ^{[1
]}

KANEMURA, T ^{[1
]}

ISONO, K ^{[1
]}

TAKEYAMA, K ^{[1
]}

TAKEUCHI, S ^{[1
]}

TAKIZAWA, T ^{[1
]}

机构：

[1] TOKYO WOMENS MED COLL,DEPT MED 1,8-1 KAWADA CHO,SHINOUKU KU,TOKYO 162,JAPAN

来源：

REGULATORY PEPTIDES | 1991年 / 34卷 / 01期

关键词：

NEUROPEPTIDE; AIRWAY; CILIUM; CYCLIC AMP; PEPTIDASE;

D O I：

10.1016/0167-0115(91)90222-3

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We studied the effect of vasoactive intestinal peptide (VIP) on ciliary activity in rabbit cultured tracheal epithelium by a photoelectric method in vitro. Administration of VIP (10(-7) M) elicited an increase in ciliary beat frequency (CBF) from the baseline values of 970 +/- 52 to 1139 +/- 75 beats/min (mean +/- S.E., P < 0.01). This ciliostimulatory effect was dose-dependent, with the maximal increase and EC50 value being 17.4 +/- 1.0 % (P < 0.05) and 6.10(-11) M, respectively. The VIP-induced increase in CBF was abolished by pretreatment of cells with [4-Cl-D-Phe6, LeU17]-VIP, a VIP receptor antagonist. The neutral endopeptidase inhibitor phosphoramidon (10(-5) M) potentiated the effect of VIP, so that the CBF dose-response curve for VIP was shifted to lower concentrations by 0.5 log U. The administration of VIP increased cyclic AMP levels in epithelial cells, an effect that was also potentiated by phosphoramidon. These results suggest that VIP may interact with its specific receptors and stimulate airway ciliary activity probably through the activation of adenylate cyclase, and that neutral endopeptidase may play a role in modulating this effect of VIP.

引用

页码：33 / 41

页数：9

共 38 条

[1] COMPARISON OF VASOACTIVE INTESTINAL PEPTIDE AND ISOPROTERENOL RELAXANT EFFECTS IN ISOLATED CAT AIRWAYS [J].

ALTIERE, RJ ;

DIAMOND, L .

JOURNAL OF APPLIED PHYSIOLOGY, 1984, 56 (04) :986-992

[2]

BORSON DB, 1986, PHYSIOLOGIST, V29, P174

[3]

CHRISTOPHE J, 1981, PEPTIDES S2, V2, P113

[4] SECRETORY ACTIONS OF VASOACTIVE INTESTINAL POLYPEPTIDE, PEPTIDE HISTIDINE ISOLEUCINE AND HELODERMIN IN RAT SMALL-INTESTINE - THE EFFECTS OF PUTATIVE VIP ANTAGONISTS UPON VIP-INDUCED ION SECRETION [J].

COX, HM ;

CUTHBERT, AW .

REGULATORY PEPTIDES, 1989, 26 (02) :127-135

[5]

DEY RD, 1981, CELL TISSUE RES, V220, P231

[6]

DIAMOND L, 1983, AM REV RESPIR DIS, V128, P827

[7] CHARACTERIZATION OF VASOACTIVE-INTESTINAL-PEPTIDE (VIP) RECEPTORS IN MAMMALIAN LUNG [J].

DICKINSON, KEJ ;

SCHACHTER, M ;

MILES, CMM ;

COY, DH ;

SEVER, PS .

PEPTIDES, 1986, 7 (05) :791-800

[8] NEUTRAL ENDOPEPTIDASE INHIBITORS POTENTIATE SUBSTANCE P-INDUCED CONTRACTION IN GUT SMOOTH-MUSCLE [J].

DJOKIC, TD ;

SEKIZAWA, K ;

BORSON, DB ;

NADEL, JA .

AMERICAN JOURNAL OF PHYSIOLOGY, 1989, 256 (01) :G39-G43

[9] HUMAN-KIDNEY ENKEPHALINASE, A NEUTRAL METALLOENDOPEPTIDASE THAT CLEAVES ACTIVE PEPTIDES [J].

GAFFORD, JT ;

SKIDGEL, RA ;

ERDOS, EG ;

HERSH, LB .

BIOCHEMISTRY, 1983, 22 (13) :3265-3271

[10] PREFERENTIAL CLEAVAGE OF AMINO-TERMINAL AND CARBOXYL-TERMINAL OLIGOPEPTIDES FROM VASOACTIVE INTESTINAL POLYPEPTIDE BY HUMAN RECOMBINANT ENKEPHALINASE (NEUTRAL ENDOPEPTIDASE, EC 3.4.24.11) [J].

GOETZL, EJ ;

SREEDHARAN, SP ;

TURCK, CW ;

BRIDENBAUGH, R ;

MALFROY, B .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 158 (03) :850-854

← 1 2 3 4 →