PROBLEMS OF ANALYZING C-18 CIS-FATTY AND TRANS-FATTY-ACIDS OF MARGARINE ON THE SP-2340 CAPILLARY COLUMN

被引：89

作者：

RATNAYAKE, WMN

BEAREROGERS, JL

机构：

[1] Bureau of Nutritional Sciences, Health Protection Branch, Health and Welfare Canada, Ottawa, ON

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1990年 / 28卷 / 12期

关键词：

D O I：

10.1093/chromsci/28.12.633

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The problems of analyzing isomeric fatty acids in partially hydrogenated oils by gas liquid chromatography (GLC) on an SP-2340 fused-capillary column are evaluated using methyl esters from margarine fatty acids and 44 synthetic C18unsaturated fatty acids. Satisfactory separations of the geometrical isomers are obtained for a given double bond system, but overlaps occur between different double bond systems and between some mono- and diethylenic, as well as between di- and triethylenic fatty acids. In margarine the major cis-octadecenoic isomers (18:1Δ6c-Δ10c) coeluted with four trans-isomers (18:1 Δ12t-Δ15t). This overlap is greater in the print type of margarine than in the tub type. Another overlap, quantitatively less important, occurs between 18:1Δ14c-Δ15c with some trans, trans-octadecadienoic acids. Because of these overlaps, the isomers cannot be determined by GLC alone on the SP-2340 liquid phase or any other cyanosilicone phase. Fractionation of fatty acids according to the degree of unsaturation via methoxybromomercuric adduct and subsequent geometrical isomer separation by silver nitrate thin-layer chromatography (TLC) prior to GLC is recommended. © 1990 Oxford University Press.

引用

页码：633 / 639

页数：7

共 40 条

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