INFLUENCE OF PLANT-GROWTH REGULATORS, BASAL MEDIA AND CARBOHYDRATE-LEVELS ON THE INVITRO DEVELOPMENT OF PINUS-PONDEROSA (DOUGL EX LAW.) COTYLEDON EXPLANTS

被引：35

作者：

TUSKAN, GA ^{[1
]}

SARGENT, WA ^{[1
]}

RENSEMA, T ^{[1
]}

WALLA, JA ^{[1
]}

机构：

[1] N DAKOTA STATE UNIV,DEPT PLANT PATHOL,FARGO,ND 58105

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1990年 / 20卷 / 01期

关键词：

callus culture; caulogenesis; in vitro screening; ponderosa pine; western gall rust;

D O I：

10.1007/BF00034756

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Applications of in vitro screening techniques for Pinus ponderosa resistance to Peridermium harknessii could be beneficial in a tree breeding program. Plant growth regulators, basal media formula and carbohydrate levels were examined to determine the various effects each would have on excised cotyledon growth and development. Proliferating green callus was initiated from cotyledon explants on SH basal medium containing 4.4 μM BA:5.4 μM NAA and 1% sucrose. Subsequent subculturing onto LS medium supplemented with 44.0 μM BA: 5.4 μM NAA and 2% sucrose improved callus maintenance. The highest frequency of caulogenesis from cotyledon explants occurred on a modified GD medium containing 44.0 μM BA: 0.054 μM NAA and 4% glucose. The influence of nitrogen source, osmoticum and medium salt concentrations are discussed relative to callus initiation and caulogenesis. © 1990 Kluwer Academic Publishers.

引用

页码：47 / 52

页数：6

共 32 条

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[2]

[Anonymous], 1991, PRINCIPLES PROCEDURE

[3]

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