ANALYSIS OF THE PROTEIN-COMPOSITION OF ALFALFA MOSAIC-VIRUS RNA-DEPENDENT RNA-POLYMERASE

被引：77

作者：

QUADT, R ^{[1
]}

ROSDORFF, HJM ^{[1
]}

HUNT, TW ^{[1
]}

JASPARS, EMJ ^{[1
]}

机构：

[1] LEIDEN STATE UNIV,GORLAEUS LABS,DEPT BIOCHEM,2300 RA LEIDEN,NETHERLANDS

来源：

VIROLOGY | 1991年 / 182卷 / 01期

关键词：

D O I：

10.1016/0042-6822(91)90674-Z

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

RNA-dependent RNA polymerase (RdRp) was solubilized and purified from cellular membranes isolated from alfalfa mosaic virus (AIMV)-infected tobacco by employing a procedure recently described for brome mosaic virus RdRp [R. Quadt and E.M.J. Jaspers 1990, Virology 178, 189-194]. The purified AIMV RdRp is completely dependent on added template RNAs and exhibits a high degree of template specificity. Analysis of the protein composition of AIMV RdRp showed that AIMV-encoded proteins P1 and P2 and the coat protein (CP) are present in the active enzyme complex. Minus-strand synthesis by the AIMV RdRp is inhibited by AIMV CP. Native double-stranded AIMV RNAs are utilized as template for viral RNA synthesis by AIMV RdRp indicating that a helicase activity is present in the purified AIMV RdRp preparation. © 1991.

引用

页码：309 / 315

页数：7

共 22 条

[1] KINETICS OF ACCUMULATION OF THE 3 NONSTRUCTURAL PROTEINS OF ALFALFA MOSAIC-VIRUS IN TOBACCO PLANTS
BERNA, A
BRIAND, JP
STUSSIGARAUD, C
GODEFROYCOLBURN, T
[J]. JOURNAL OF GENERAL VIROLOGY, 1986, 67 : 1135 - 1147
[2] BERNA A, 1984, ANN VIROL E, V135, P283
[3] ISOLATION OF VIRAL DOUBLE-STRANDED RNAS USING A LICL FRACTIONATION PROCEDURE
DIAZRUIZ, JR
KAPER, JM
[J]. PREPARATIVE BIOCHEMISTRY, 1978, 8 (01): : 1 - 17
[4] GOLDBACH R, 1987, MICROBIOL SCI, V4, P197
[5] VIRAL-PROTEINS CONTAINING THE PURINE NTP-BINDING SEQUENCE PATTERN
GORBALENYA, AE
KOONIN, EV
[J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (21) : 8413 - 8440
[6] COMPLETE REPLICATION OF A EUKARYOTIC VIRUS-RNA INVITRO BY A PURIFIED RNA-DEPENDENT RNA-POLYMERASE
HAYES, RJ
BUCK, KW
[J]. CELL, 1990, 63 (02) : 363 - 368
[7] BROME MOSAIC-VIRUS COAT PROTEIN INHIBITS VIRAL-RNA SYNTHESIS INVITRO
HORIKOSHI, M
NAKAYAMA, M
YAMAOKA, N
FURUSAWA, I
SHISHIYAMA, J
[J]. VIROLOGY, 1987, 158 (01) : 15 - 19
[8] PREFERENTIAL BINDING OF 3'-TERMINAL FRAGMENTS OF ALFALFA MOSAIC-VIRUS RNA-4 TO VIRIONS
HOUWING, CJ
JASPARS, EMJ
[J]. BIOCHEMISTRY, 1980, 19 (23) : 5261 - 5264
[9] COAT PROTEIN BLOCKS THE INVITRO TRANSCRIPTION OF THE VIRION RNAS OF ALFALFA MOSAIC-VIRUS
HOUWING, CJ
JASPARS, EMJ
[J]. FEBS LETTERS, 1986, 209 (02) : 284 - 288
[10] INVITRO EVIDENCE THAT THE COAT PROTEIN IS THE PROGRAMMING FACTOR IN ALFALFA MOSAIC VIRUS-INDUCED RNA-SYNTHESIS
HOUWING, CJ
JASPARS, EMJ
[J]. FEBS LETTERS, 1987, 221 (02) : 337 - 342

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