REGULATION AND EXPRESSION OF TRANSFORMING GROWTH-FACTOR TYPE-BETA DURING EARLY MAMMALIAN DEVELOPMENT

被引：35

作者：

KELLY, D ^{[1
]}

CAMPBELL, WJ ^{[1
]}

TIESMAN, J ^{[1
]}

RIZZINO, A ^{[1
]}

机构：

[1] UNIV NEBRASKA,MED CTR,EPPLEY INST CANC RES & ALLIED DIS,42ND & DEWEY AVE,OMAHA,NE 68198

来源：

CYTOTECHNOLOGY | 1990年 / 4卷 / 03期

关键词：

TRANSFORMING GROWTH FACTOR TYPE-BETA; EMBRYONAL CARCINOMA CELLS; MAMMALIAN EMBRYOGENESIS; POLYMERASE CHAIN REACTION;

D O I：

10.1007/BF00563783

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have examined the effect of differentiation on the expression of different members of the transforming growth factor type-beta (TGF-B) family using embryonal carcinoma (EC) cells and early mammalian embryos. We determined that TGF-B activity increases approximately 25-100% when the mouse EC cell line, F9, is induced to differentiate with retinoic acid (RA). Interestingly, the increased TGF-B activity reflects the induction of TGF-B2 secretion following differentiation of both F9 EC cells and the human EC cell line, NT2/D1. Using the technique of reverse transcription-polymerase chain reaction (RT-PCR), we have verified that differentiation induces the expression of TGF-B2 as well as a distant member of the TGF-B family, Vgr-1. Transcripts for TGF-B2 and Vgr-1 were readily detected in the differentiated cells of F9 and PC-13 but not in their undifferentiated counterparts. Moreover, TGF-B2 mRNA was readily detected in NT2/D1 cells following differentiation. In addition, transcripts for TGF-B2 were detected by RT-PCR in mouse morulae, preimplantation blastocysts and cultured blastocysts. Based on the data presented, it appears that the expression of both TGF-B2 and Vgr-1 is closely associated with the induction of differentiation during early development.

引用

页码：227 / 242

页数：16

共 49 条

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