VOLTAGE-DEPENDENT AND TIME-DEPENDENT INHIBITION OF NEURONAL CALCIUM CHANNELS BY A GTP-BINDING PROTEIN IN A MAMMALIAN-CELL LINE

被引:109
作者
KASAI, H [1 ]
机构
[1] MAX PLANCK INST BIOPHYS CHEM,MEMBRANBIOPHYS ABT,W-3400 GOTTINGEN,GERMANY
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1992年 / 448卷
关键词
D O I
10.1113/jphysiol.1992.sp019036
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. Inhibitory modulation of the three Ca2+ channel current components by neurotransmitters was studied using the whole-cell patch-clamp method in a mammalian cell line, NG108-15. 2. In cells differentiated with dibutyryl cyclic AMP, both the low-voltage-activated current (I(LVA)) and omega-CgTX-sensitive high-voltage-activated current (I(omega-CgTX)) could be inhibited by [D-phen2, D-phen5]enkephalin, acetylcholine and noradrenaline. In contrast, differentiation with prostaglandin E1 and theophylline eliminated the agonist-induced inhibition of I(LVA), but enhanced that of I(omega-CgTX). The DHP-sensitive high-voltage-activated current was unaffected by the transmitters in most of the cells. 3. The inhibition was prevented by pre-treatment of cells with pertussis toxin, suggesting involvement of a G-protein. Long treatment of the cells with phorbol ester did not prevent the inhibition. 4. The inhibition was always partial: the maximal inhibition was 40% for I(LVA) and 70% for I(omega-CgTX). 5. The inhibition of I(LVA) and I(omega-CgTX) was relieved during depolarization. Half-maximal relief of inhibition of I(omega-CgTX) was attained at 0 mV, irrespective of agonist concentration. 6. The kinetics of removal and re-establishment of inhibition were voltage dependent. Both processes were single exponentials and had identical time constants at a given membrane potential. Time constants were 124 ms at -40 mV, 160 ms at 0 mV and 8 ms at 60 mV, at any agonist concentration. 7. Time courses of tail currents were unaltered by the inhibition. 8. The inhibition of the omega-CgTX-sensitive Ca2+ channel can be described as a shift in gating modes; with an additional voltage-dependent gating state activated by the agonists. The voltage-dependent properties of this modulation allow inhibition of Ca2+ channel to be overcome by high-frequency trains of action potentials.
引用
收藏
页码:189 / 209
页数:21
相关论文
共 44 条
[1]   CHARACTERIZATION OF 2 KINDS OF HIGH-VOLTAGE-ACTIVATED CA-CHANNEL CURRENTS IN CHICK SENSORY NEURONS - DIFFERENTIAL SENSITIVITY TO DIHYDROPYRIDINES AND OMEGA-CONOTOXIN GVIA [J].
AOSAKI, T ;
KASAI, H .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1989, 414 (02) :150-156
[2]   ACTIVATION OF FACILITATION CALCIUM CHANNELS IN CHROMAFFIN CELLS BY D1 DOPAMINE-RECEPTORS THROUGH A CAMP PROTEIN KINASE-A-DEPENDENT MECHANISM [J].
ARTALEJO, CR ;
ARIANO, MA ;
PERLMAN, RL ;
FOX, AP .
NATURE, 1990, 348 (6298) :239-242
[4]   CONTINUOUS SYNTHESIS OF 2 PROTEIN-KINASE C-RELATED PROTEINS AFTER DOWN-REGULATION BY PHORBOL ESTERS [J].
BORNER, C ;
EPPENBERGER, U ;
WYSS, R ;
FABBRO, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (07) :2110-2114
[5]   NEURONAL CALCIUM CHANNELS - KINETICS, BLOCKADE AND MODULATION [J].
CARBONE, E ;
SWANDULLA, D .
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1989, 54 (01) :31-58
[6]   SEROTONIN DECREASES POPULATION SPIKE AMPLITUDE IN HIPPOCAMPAL CELLS THROUGH A PERTUSSIS TOXIN SUBSTRATE [J].
CLARKE, WP ;
DEVIVO, M ;
BECK, SG ;
MAAYANI, S ;
GOLDFARB, J .
BRAIN RESEARCH, 1987, 410 (02) :357-361
[7]   PERTUSSIS TOXIN BLOCKS DEPRESSANT EFFECTS OF OPIOID, MONOAMINERGIC AND MUSCARINIC AGONISTS ON DORSAL-HORN NETWORK RESPONSES IN SPINAL CORD-GANGLION CULTURES [J].
CRAIN, SM ;
CRAIN, B ;
MAKMAN, MH .
BRAIN RESEARCH, 1987, 400 (01) :185-190
[8]   GAMMA-AMINOBUTYRIC ACID-INDUCED DEPRESSION OF CALCIUM CURRENTS OF CHICK SENSORY NEURONS [J].
DEISZ, RA ;
LUX, HD .
NEUROSCIENCE LETTERS, 1985, 56 (02) :205-210
[9]   NORADRENALINE-INDUCED INHIBITION OF VOLTAGE-SENSITIVE CALCIUM CURRENTS IN NG108-15 HYBRID-CELLS [J].
DOCHERTY, RJ ;
MCFADZEAN, I .
EUROPEAN JOURNAL OF NEUROSCIENCE, 1989, 1 (02) :132-140
[10]  
DOLPHIN AC, 1987, J PHYSIOL-LONDON, V386, P1