TRANSCRIPTION OF THE CHICKEN ANEMIA VIRUS (CAV) GENOME AND SYNTHESIS OF ITS 52-KDA PROTEIN

被引：53

作者：

NOTEBORN, MHM ^{[1
]}

KRANENBURG, O ^{[1
]}

ZANTEMA, A ^{[1
]}

KOCH, G ^{[1
]}

DEBOER, GF ^{[1
]}

VANDEREB, AJ ^{[1
]}

机构：

[1] CENT VET INST,DEPT VIROL,LELYSTAD,NETHERLANDS

来源：

GENE | 1992年 / 118卷 / 02期

关键词：

RNA TRANSCRIPT; POLYCISTRONIC; OVERLAPPING ORFS; NORTHERN BLOT; DOT-BLOT ANALYSIS; S1; MAPPING; INVITRO TRANSLATION;

D O I：

10.1016/0378-1119(92)90198-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

This paper describes the expression of the chicken anemia virus (CAV) genome, a recently characterized single-stranded circular-DNA virus of a new type [Noteborn et al., J. Virol. 65 (1991) 3131-3139]. The major transcript from the CAV genome is an unspliced mRNA of about 2100 nucleotides (nt). Its transcription start point and poly(A)-addition site are located at nt 354 and 2317 of the CAV sequence, respectively. In vitro translation experiments provide evidence that the major CAV open reading frame encodes a 52-kDa protein by using the fifth AUG as a start codon of the unspliced CAV mRNA.

引用

页码：267 / 271

页数：5

共 16 条

[1] MORPHOLOGICAL CHARACTERIZATION OF CHICKEN ANEMIA AGENT (CAA) [J].