SURFACE CHARACTERIZATION OF MUSSEL ADHESIVE PROTEIN FILMS

被引：29

作者：

OLIVIERI, MP ^{[1
]}

LOOMIS, RE ^{[1
]}

MEYER, AE ^{[1
]}

BAIER, RE ^{[1
]}

机构：

[1] NEW YORK STATE DEPT HLTH,ROSWELL PK MEM INST,DEPT BIOPHYS,BUFFALO,NY 14263

来源：

JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY | 1990年 / 4卷 / 03期

关键词：

Bioadhesives; mussel adhesive protein; protein adsorption; thin films;

D O I：

10.1163/156856190X00216

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Mussel adhesive protein (MAP) from the marine mussel Mytilus edulis is considered to be responsible for attaching it to surfaces in its native environment. The isolated and partially purified form of MAP, marketed as ’Ceil-Tak™’ (BioPolymers, Inc., Farmington, CT), is currently being used as an in vitro aid for attachment of cells to culture vessels. The collective results reported here indicate that Cell-Tak™ binds strongly to clean germanium plates, serving as surrogate substrata for those found in many natural situations. These ’conditioning films’ become thicker with increasing applied concentration, which is typical of most proteins. This effectively masks the physical-chemical properties of the original solid surface from the cells, while expressing new adhesive properties for cell attachment, which might well be a recognition phenomenon related to specific functional groups and/or surface configuration of the protein. The data presented here show that polar surface energy component (yp) values of 12־־ dynes/cm, in conjunction with critical surface tensions above -30 dynes/cm, are associated with concentrations near 2 ßg/cm2 for maximal cell attachment. © 1990, VSP. All rights reserved.

引用

页码：197 / 204

页数：8

共 15 条

[1]

Waite J.H., Tanzer M.L., Science, 212, pp. 1038-1040, (1981)

[2]

Waite J.H., J. Biol. Chem., 258, pp. 2911-2915, (1983)

[3]

Biopolymers I., Farmington C.T., Cell-Talix, Update No. 1, (1986)

[4]

Biopolymers I., Farmington C.T., Cell-Tak, Update No. 2, (1987)

[5]

Biopolymers I., Farmington C.T., Cell-Tak, Update No. 3, (1988)

[6]

Mccrackin F.L., Colson J.P., ELIP: A Fortran Program for Analyses of Ellipsometer Measurements and Calculation of Reflection Coefficients from, Thin Films. National Bureau of Standards, Misc. Pub., 256, (1964)

[7]

Baier R.E., Meyer A.E., Handbook of Biomaterials Evaluation: Scientific, Technical and Clinical Testing of Implant, Materials, pp. 97-108, (1986)

[8]

Baier R.E., Gott V.L., Feruse A., Am. Soc. Artif Int., 16, pp. 50-57, (1970)

[9]

Zisman W.A., Adv. Chem. Ser., 43, pp. 1-51, (1964)

[10]

Kaelble D.H., J. Phys. Chem., 64, pp. 1110-1114, (1970)

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