LOCALIZATION OF MURINE IG-1B AND IG-1A (IGG 2A) ALLOTYPIC DETERMINANTS DETECTED WITH MONOCLONAL ANTIBODIES

We have used hybridoma anti-allotype antibodies to define six allotypic determinants: four on Ig-1b molecules and two on Ig-1a molecules. By locating these determinants on fragments obtained by limited proteolysis of IgG2a, molecules and by hybridoma antibody blocking assays, five distinct allotypic sites have been defined on mouse γ2a heavy chains. These sites are located in the hinge region and the CH2 and CH3 domains. We have not been able to show genetic recombination between these sites by genetic linkage studies. © 1979.