ANALYSIS OF THE GENES ENCODING IMMUNODOMINANT PIROPLASM SURFACE-PROTEINS OF THEILERIA-SERGENTI AND THEILERIA-BUFFELI BY NUCLEOTIDE SEQUENCING AND POLYMERASE CHAIN-REACTION

被引：66

作者：

KAWAZU, S ^{[1
]}

SUGIMOTO, C ^{[1
]}

KAMIO, T ^{[1
]}

FUJISAKI, K ^{[1
]}

机构：

[1] HOKKAIDO UNIV, FAC VET MED, SAPPORO, HOKKAIDO 060, JAPAN

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1992年 / 56卷 / 01期

关键词：

NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PIROPLASM; THEILERIA-BUFFELI; THEILERIA-ORIENTALIS; THEILERIA-SERGENTI;

D O I：

10.1016/0166-6851(92)90164-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nucleotide sequences of the cDNAs encoding a 33-kDa piroplasm protein of Theileria sergenti (p33) and a similar protein of Theileria buffeli (p34) were determined. Both of the genes contained an open reading frame of 849 base pairs. The predicted amino acid sequence of p33 and p34, consisting of 283 residues, showed 82% similarity. A transmembrane hydrophobic domain and signal peptides were predicted. The polymerase chain reaction was used to amplify p33/34 genes from the piroplasm DNA of T. sergenti, T. buffeli and Theileria orientalis. Following amplification, p33 and p34 genes were clearly differentiated using the restriction enzymes sites that were not shared between them. These results indicated that p33 and p34 were conserved molecules among these Theileria species, and the genes that encode p33/34 proteins were suitable for discrimination of T. sergenti from T. buffeli/T. orientalis.

引用

页码：169 / 176

页数：8

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