STIMULATION OF PHOSPHOLIPASE-A2 BY XANTHINE-OXIDASE IN THE RAT CORPUS-LUTEUM

被引：18

作者：

WU, XM ^{[1
]}

SAWADA, M ^{[1
]}

CARLSON, JC ^{[1
]}

机构：

[1] UNIV WATERLOO,DEPT BIOL,WATERLOO N2L 3G1,ONTARIO,CANADA

来源：

BIOLOGY OF REPRODUCTION | 1992年 / 47卷 / 06期

关键词：

D O I：

10.1095/biolreprod47.6.1053

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The ability of the superoxide radical (SOR) generated by xanthine oxidase to activate phospholipase A2 (PLA2) was examined in microsomes prepared from luteinized rat ovaries. Treatment of microsomes with xanthine oxidase resulted in a rapid burst in SOR formation followed by an increase in PLA2 activity. Stimulation of PLA2 activity was dose related and similar in microsomes prepared from control or prostaglandin F2alpha (PGF2alpha)-treated rats. Activation was inhibited by the antioxidants, vitamin E and nordihydrogualaretic acid, and by superoxide dismutase and catalase, which metabolize SOR and H2O2 to remove reactive oxygen species from the cell. The stimulation of PLA2 activity by xanthine oxidase was dependent upon the addition of calcium ions, and it was highest in samples in which cytosol was added to membranes. These results indicate that the SOR and/or H2O2 may mediate PIA, activation, which may be involved in the luteolytic process.

引用

页码：1053 / 1058

页数：6

共 28 条

[1] REGULATION OF OVARIAN ANTIOXIDANT VITAMINS, REDUCED GLUTATHIONE, AND LIPID-PEROXIDATION BY LUTEINIZING-HORMONE AND PROSTAGLANDIN-F2-ALPHA [J].