COMPARISON OF WESTERN-BLOT AND MICROIMMUNOFLUORESCENCE AS TOOLS FOR LYME-DISEASE SEROEPIDEMIOLOGY

被引：12

作者：

ARZOUNI, JP ^{[1
]}

LAVERAN, M ^{[1
]}

BEYTOUT, J ^{[1
]}

RAMOUSSE, O ^{[1
]}

RAOULT, D ^{[1
]}

机构：

[1] FAC MED MARSEILLE,UNITE RICKETTSIES,27 BLVD JEAN MOULIN,F-13385 MARSEILLE 05,FRANCE

来源：

EUROPEAN JOURNAL OF EPIDEMIOLOGY | 1993年 / 9卷 / 03期

关键词：

BORRELIA-BURGDORFERI; LYME DISEASE; WESTERN BLOT; SEROEPIDEMIOLOGY;

D O I：

10.1007/BF00146262

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Sera from a population of 212 farmers of the Puy de Dome (Midlands of France) who are in close contact with Ixodes ricinus, the vector of Lyme disease in Europe, and sera from 100 urban blood donors from the nearly city of Clermont Ferrand were examined by microimmunofluorescence (MIF) for antibodies reacting with Borrelia burgdorferi. The results showed a higher seroprevalence of IgG > 1/100 in farmers (25%) than in blood donors (10%). Using western blot with antibody at a 1/200 dilution, and regarding sera as being positive if they contained IgG reactive with more than 5 bands, 93/212 farmers (44%) and 20% of blood donors were positive. Reactions with specific protein bands (94, 73, 30 and 21 KDa) by western blot were demonstrated in 83/212 sera from farmers (39%) and 16% of blood donors. Both methods showed a higher seroprevalence in the farmer population. Western blot is a sensitive and specific test for seroepidemiology but, in highly endemic areas, it is not diagnostic for active Lyme borrelliosis.

引用

页码：269 / 273

页数：5

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