FLUORESCENCE IN-SITU HYBRIDIZATION ANALYSIS OF CHROMOSOMAL LOCALIZATION OF 3 HUMAN CYTOCHROME-P450-2C GENES (CYP2C8, 2C9, AND 2C10) AT 10Q24.1

被引：18

作者：

INOUE, K

INAZAWA, J

SUZUKI, Y

SHIMADA, T

YAMAZAKI, H

GUENGERICH, FP

ABE, T

机构：

[1] KYOTO PREFECTURAL UNIV MED,DEPT HYG,KAMIGYO KU,KYOTO 602,JAPAN

[2] VANDERBILT UNIV,SCH MED,DEPT BIOCHEM,NASHVILLE,TN 37232

[3] VANDERBILT UNIV,SCH MED,CTR MOLEC TOXICOL,NASHVILLE,TN 37232

来源：

JAPANESE JOURNAL OF HUMAN GENETICS | 1994年 / 39卷 / 03期

关键词：

FLUORESCENCE IN SITU HYBRIDIZATION; CYP2C SUBFAMILY; CHROMOSOME; 10; CHROMOSOMAL LOCALIZATION; POLYMERASE CHAIN REACTION;

D O I：

10.1007/BF01874052

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Chromosomal localization of three human cytochrome P450 genes belonging to the CYP2C subfamily (CYP2C8, 2C9, and 2C10) was identified by fluorescence in situ hybridization (FISH). An original MP-8 clone was used as a DNA probe for the assignment of the CYP2C10 gene, while two cDMA probes, a 1.37 kb fragment of CYP2C8 and a 1.19 kb fragment of CYP2C9, were obtained after amplifying the predicted fragments (MP-20 and MP-4 clones, respectively) by polymerase chain reaction using a single human liver cDNA library. The results showed that three human CYP2C8, 2C9, and 2C10 cDNAs were located at the same subchromosomal region, 10q24.1.

引用

页码：337 / 343

页数：7

共 20 条

[1] EXPRESSION OF A HUMAN-LIVER CYTOCHROME-P-450 PROTEIN WITH TOLBUTAMIDE HYDROXYLASE-ACTIVITY IN SACCHAROMYCES-CEREVISIAE
BRIAN, WR
SRIVASTAVA, PK
UMBENHAUER, DR
LLOYD, RS
GUENGERICH, FP
[J]. BIOCHEMISTRY, 1989, 28 (12) : 4993 - 4999
[2] CHARACTERIZATION OF CDNAS, MESSENGER-RNAS, AND PROTEINS RELATED TO HUMAN-LIVER MICROSOMAL CYTOCHROME-P-450 (S)-MEPHENYTOIN 4'-HYDROXYLASE
GED, C
UMBENHAUER, DR
BELLEW, TM
BORK, RW
SRIVASTAVA, PK
SHINRIKI, N
LLOYD, RS
GUENGERICH, FP
[J]. BIOCHEMISTRY, 1988, 27 (18) : 6929 - 6940
[3] ASSIGNMENT OF THE HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR RECEPTOR GENE (CSF3R) TO CHROMOSOME-1 AT REGION P35-P34.3
INAZAWA, J
FUKUNAGA, R
SETO, Y
NAKAGAWA, H
MISAWA, S
ABE, T
NAGATA, S
[J]. GENOMICS, 1991, 10 (04) : 1075 - 1078
[4] ASSIGNMENT OF THE HUMAN CYTOCHROME-P-450 NIFEDIPINE OXIDASE GENE (CYP3A4) TO CHROMOSOME-7 AT BAND Q22.1 BY FLUORESCENCE INSITU HYBRIDIZATION
INOUE, K
INAZAWA, J
NAKAGAWA, H
SHIMADA, T
YAMAZAKI, H
GUENGERICH, FP
ABE, T
[J]. JAPANESE JOURNAL OF HUMAN GENETICS, 1992, 37 (02): : 133 - 138
[5] PHARMACOGENETICS OF MEPHENYTOIN - A NEW DRUG HYDROXYLATION POLYMORPHISM IN MAN
KUPFER, A
PREISIG, R
[J]. EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY, 1984, 26 (06) : 753 - 759
[6] A SIMPLE METHOD FOR SIMULTANEOUS R-BANDING OR G-BANDING AND FLUORESCENCE INSITU HYBRIDIZATION OF SMALL SINGLE-COPY GENES
LEMIEUX, N
DUTRILLAUX, B
VIEGASPEQUIGNOT, E
[J]. CYTOGENETICS AND CELL GENETICS, 1992, 59 (04): : 311 - 312
[7] MEEHAN RR, 1988, AM J HUM GENET, V42, P26
[8] THE P450 SUPERFAMILY - UPDATE ON NEW SEQUENCES, GENE-MAPPING, ACCESSION NUMBERS, EARLY TRIVIAL NAMES OF ENZYMES, AND NOMENCLATURE
NELSON, DR
KAMATAKI, T
WAXMAN, DJ
GUENGERICH, FP
ESTABROOK, RW
FEYEREISEN, R
GONZALEZ, FJ
COON, MJ
GUNSALUS, IC
GOTOH, O
OKUDA, K
NEBERT, DW
[J]. DNA AND CELL BIOLOGY, 1993, 12 (01) : 1 - 51
[9] OKINO ST, 1987, J BIOL CHEM, V262, P16072
[10] CYTOGENETIC ANALYSIS USING QUANTITATIVE, HIGH-SENSITIVITY, FLUORESCENCE HYBRIDIZATION
PINKEL, D
STRAUME, T
GRAY, JW
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (09) : 2934 - 2938

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