A SELECTIVE AUXIN AND CYTOKININ BIOASSAY BASED ON ROOT AND SHOOT FORMATION IN-VITRO

被引:9
作者
GYULAI, G
KISS, J
JEKKEL, Z
KISS, E
HESZKY, LE
机构
[1] Department of Genetics and Plant Breeding, University of Agricultural Sciences, Godoiis
基金
匈牙利科学研究基金会;
关键词
BIOASSAY; PLANT GROWTH REGULATORS; SELECTIVITY; TISSUE CULTURE;
D O I
10.1016/S0176-1617(11)81907-3
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A new bioassay for auxins and cytokinins was developed based on de novo organogenesis. Auxin-dependent adventitious roots and cytokinin-dependent adventitious shoots were induced selectively in tobacco leaf disc culture incubated on nutritive-medium for 28 days. All auxins applied alone (indole, naphthalene, and phenoxyacetic acids) initiated roots, and all cytokinins used alone (purine and urea derivatives) initiated shoots at a concentration range of about 10(-9) M-10(-4) M. The bioassay separated the effects of auxins and cytokinins eliminating their synergistic effects. Abscisic acid and gibberellin (GA(3)) had no morphogenic activity.**
引用
收藏
页码:379 / 382
页数:4
相关论文
共 19 条
[1]   APPLICATION OF THE DISK METHOD - RESPONSES TO GROWTH-REGULATORS [J].
ANDERSON, DR ;
CAMPER, ND .
JOURNAL OF PLANT GROWTH REGULATION, 1987, 6 (01) :57-65
[2]   RESTORATION OF THE REGENERATION POTENTIAL OF LONG-TERM CELL-CULTURE IN RICE (ORYZA-SATIVA L) BY SALT PRETREATMENT [J].
BINH, DQ ;
HESZKY, LE .
JOURNAL OF PLANT PHYSIOLOGY, 1990, 136 (03) :336-340
[3]  
DUDITS D, 1991, J CELL SCI, V99, P473
[4]   COTYLEDON EXPANSION AS A BIOASSAY FOR CYTOKININS [J].
ESASHI, Y ;
LEOPOLD, AC .
PLANT PHYSIOLOGY, 1969, 44 (04) :618-&
[5]   ACTIVITY OF SOME 6- (SUBSTITUTED) PURINES ON THE DEVELOPMENT OF THE MOSS TORTELLA-CAESPITOSA [J].
GORTON, BS ;
SKINNER, CG ;
EAKIN, RE .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1957, 66 (02) :493-496
[6]   A CYTOKININ TEST WITH HIGH SPECIFICITY [J].
HAHN, H ;
BOPP, M .
PLANTA, 1968, 83 (01) :115-&
[7]  
HALMANN M, 1990, ADV AGRON, V43, P47
[8]   ORGANIC GROWTH FACTOR REQUIREMENTS OF TOBACCO TISSUE CULTURES [J].
LINSMAIER, EM ;
SKOOG, F .
PHYSIOLOGIA PLANTARUM, 1965, 18 (01) :100-+
[9]  
LORZ H, 1985, MOL GEN GENET, V199, P178, DOI 10.1007/BF00330256
[10]   NON-MENDELIAN STREPTOMYCIN-RESISTANT TOBACCO MUTANT WITH ALTERED CHLOROPLASTS AND MITOCHONDRIA [J].
MALIGA, P ;
SZBREZNOVITS, A ;
MARTON, L ;
JOO, F .
NATURE, 1975, 255 (5507) :401-402