FIRST DIRECT INTERFACIAL ELECTRON-TRANSFER BETWEEN A BIOMOLECULE AND A SOLID ELECTRODE IN NONAQUEOUS MEDIA - DIRECT ELECTROCHEMISTRY OF MICROPEROXIDASE-11 AT GLASSY-CARBON IN DIMETHYL-SULFOXIDE SOLUTION

被引：26

作者：

MABROUK, PA

机构：

[1] Department of Chemistry, Northeastern University, Boston

来源：

ANALYTICA CHIMICA ACTA | 1995年 / 307卷 / 2-3期

关键词：

BIOELECTROCHEMISTRY; CYCLIC VOLTAMMETRY; ENZYMATIC METHODS; MICROPEROXIDASE;

D O I：

10.1016/0003-2670(94)00559-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The direct heterogeneous electron transfer between the imidazole complex of microperoxidase-11 (im-MP-11) and naked glassy carbon electrodes in dimethyl sulfoxide (1-3.6% H2O) containing 0.1 M tetra-n-butylammonium perchlorate has been accomplished using cyclic voltammetry. Homogeneous solutions of im-MP-11 in dimethyl sulfoxide were prepared by weak complexation with 18-crown-6. Heterogeneous electron transfer between im-MP-11 and glassy carbon has been shown to be persistent and at least quasi-reversible in dimethyl sulfoxide solution. The redox potential for im-MP-11 in dimethyl sulfoxide has been found to be - 274 +/- 5 mV vs. Ag/AgCl - significantly less negative than the potential (- 389 mV vs. Ag/AgCl) for this same complex in aqueous 75 mM phosphate buffer, pH 7.0, containing 0.1 M NaClO4. The calculated diffusion coefficient (1.8 X 10(-7) cm(2) s(-1)) and the rate of electron transfer in dimethyl sulfoxide (2.7 +/- 0.3 X 10(-4) cm s(-1)) are approximately one order of magnitude lower than that for the same complex in 0.02 M aqueous phosphate buffer, pH 7.0.

引用

页码：245 / 251

页数：7

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