CLONING OF CLOSTRIDIUM THERMOCELLUM ENDOGLUCANASE GENES IN ESCHERICHIA-COLI

被引：23

作者：

BUMAZKIN, BK

VELIKODVORSKAYA, GA

TUKA, K

MOGUTOV, MA

STRONGIN, AY

机构：

[1] ACAD SCI USSR,INST MOLEC GENET,KURCHATOV SQ,MOSCOW 123182,USSR

[2] ACAD SCI USSR,CENDISI BIOPROC,MOSCOW 121069,USSR

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 167卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)90630-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Six independent and distinct cel genes coding endoglucanases have been selected from C. thermocellum pUC19-based gene bank in E.coli TG1. E.coli-derived Cel-proteins possessing Mr from 39,000 to 61,000 are able to cleave lichenan, as well as xylan and carboxymethyl cellulose. Cel 7- and Cel 8-endoglucanases are characterized by cellobiohydrolase type substrate specificity, being able to cleave model fluorogenic aryldisaccharide substrate MU-G2. The clone pCU110 (cel 7) produces about 10-fold more endoglucanase activity than other clones. © 1990.

引用

页码：1057 / 1064

页数：8

共 22 条

[1]

BEGUIN P, 1985, J BACTERIOL, V162, P102

[2]

BEGUIN P, 1982, ANAL BIOCHEM, V2131, P333

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4]

Chuvilskaya N. A., 1986, APPL BIOCHEM MICRO+, V22, P800