APPLICATION OF SCREENING AND CONFIRMATORY ASSAYS FOR ANTI-HTLV-I/II IN UK POPULATIONS

被引：15

作者：

TOSSWILL, JHC ^{[1
]}

PARRY, JV ^{[1
]}

WEBER, JN ^{[1
]}

机构：

[1] ST MARYS HOSP,LONDON,ENGLAND

来源：

JOURNAL OF MEDICAL VIROLOGY | 1992年 / 36卷 / 03期

关键词：

VIRAL DIAGNOSTICS; HTLV SCREENING; HTLV CONFIRMATION; ANTENATAL SCREENING;

D O I：

10.1002/jmv.1890360304

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

During an epidemiological study of a low risk U.K. population diverse screening and confirmatory assays for the detection of anti-HTLV-I/II were assessed. Sera from 2,900 antenatal patients were tested for anti-HTLV-I/II by gelatin particle agglutination assay (GPA). All reactive sera, and 133 randomly selected unreactive sera, were further tested by Abbott and DuPont ELISAs, "in house" competitive and IgG capture radioimmunoassays (RIAs), and Western blot (WB). Sera which reacted with any HTLV-I proteins by WB were tested by radio immunoprecipitation assays (RIPA). The two ELISAs detected all the GPA reactive specimens that were subsequently confirmed as anti-HTLV-I/II positive. Confirmation of positive screening results required the use of both WB and RIPA. Serological diagnosis of HTLV infection involves access to specialised assays that are not commercially available.

引用

页码：167 / 171

页数：5

共 21 条

[1] RETROSPECTIVE STUDY OF HIV, HEPATITIS-B, AND HTLV-I INFECTION AT A LONDON ANTENATAL CLINIC [J].