INHIBITION OF MITOCHONDRIAL RESPIRATION BY UNCOUPLERS OF OXIDATIVE PHOSPHORYLATION .2. SITE OF INHIBITION OF SUCCINATE OXIDATIVE BY UNCOUPLERS

被引：23

作者：

WILSON, DF

MERZ, R

机构：

[1] Department of Biophysics and Physical Biochemistry, Johnson Research Foundation, University of Pennsylvania, Philadelphia

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 129卷 / 01期

关键词：

D O I：

10.1016/0003-9861(69)90153-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dicumarol, an uucoupler of mitochondrial oxidative phosphorylation, inhibits the oxidation of succinate by rat liver mitochondria and submitochondrial particles. This inhibition is competitive with respect to succinate and the second power with respect to dicumarol in both systems. The respective inhibitor constants are approximately 4 × 102 μm2 for intact mitochondria (State 3), 10 × 102102μm2 for submitochondrial particles prepared by sonication, and 45 × 102μm2 for succinate-cytochrome c reductase particles. The uncouplers dinitrophenol and the m-chlorophenylhydrazone of carbonyl cyanide also inhibit succinate oxidation in submitochondrial particles in a manner consistant with their inhibition of succinate oxidation in intact mitochondria. It is concluded that all of these uncouplers inhibit succinate oxidation by directly inhibiting the succinate dehydrogenase. © 1969.

引用

页码：79 / &

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