MEMBRANE-BOUND HYDROGENASE OF ALCALIGENES-EUTROPHUS .1. SOLUBILIZATION, PURIFICATION, AND BIOCHEMICAL-PROPERTIES

被引:197
作者
SCHINK, B [1 ]
SCHLEGEL, HG [1 ]
机构
[1] GESELL STRAHLEN & UMWELTFORSCH MBH, D-3400 GOTTINGEN, GERMANY
关键词
(Alcaligenes eutrophus); Hydrogenase; Iron-sulfur protein; Membrane bound;
D O I
10.1016/0005-2744(79)90117-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The membrane-bound hydrogenase of Alcaligenes eutrophus was solubilized from washed membranes of autotrophically grown cells. The enzyme consists of two types of subunits and is an iron-sulfur protein. A flavin compound was not detected. The enzyme reacts only with few artificial electron acceptors. © 1979.
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页码:315 / 324
页数:10
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