SIMULTANEOUS PRODUCTION OF GLUCOSE-OXIDASE AND CATALASE BY ALTERNARIA-ALTERNATA

被引：6

作者：

CARIDIS, KA ^{[1
]}

CHRISTAKOPOULOS, P ^{[1
]}

MACRIS, BJ ^{[1
]}

机构：

[1] NATL TECH UNIV ATHENS,DEPT CHEM ENGN,9 IROON POLYTECH AVE,POLYTECHNIOUPOLIS ZOGRAFOU,GR-15773 ATHENS,GREECE

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1991年 / 34卷 / 06期

关键词：

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A number of factors affecting simultaneous production of cell-bound glucose oxidase and catalase by the fungus Alternaria alternata have been investigated. Consecutive optimization of the type and concentration of nitrogen and carbon source, the initial pH and growth temperature resulted in a simultaneous increase in glucose oxidase and catalase by 780% and 68% respectively. Two second-order equations, describing the combined effect of pH and temperature on the activity of each enzyme, revealed that glucose oxidase had its optima at pH 7.9 and 32.3-degrees-C and catalase at pH 8.5 and 18.1-degrees-C. Under certain growth conditions, yields as high as 23.5 and 18,100 units/g carbon source for glucose oxidase and catalase, respectively, were simultaneously obtained.

引用

页码：794 / 797

页数：4

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