SUITABILITY OF THE PRFA GENE, WHICH ENCODES A REGULATOR OF VIRULENCE GENES IN LISTERIA-MONOCYTOGENES, IN THE IDENTIFICATION OF PATHOGENIC LISTERIA SPP

被引：48

作者：

WERNARS, K

HEUVELMAN, K

NOTERMANS, S

DOMANN, E

LEIMEISTERWACHTER, M

CHAKRABORTY, T

机构：

[1] NATL INST PUBL HLTH & ENVIRONM PROTECT, 3720 BA BILTHOVEN, NETHERLANDS

[2] UNIV WURZBURG, INST GENET & MIKROBIOL, W-8700 WURZBURG, GERMANY

[3] UNIV WURZBURG, MED KLIN, BIOCHEM KLIN, W-8700 WURZBURG, GERMANY

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 02期

关键词：

D O I：

10.1128/AEM.58.2.765-768.1992

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The pathogenesis of listerial infections is complex and involves a number of virulence factors expressed by virulent Listeria species. We have recently described a regulator gene, prfA, that positively regulates the expression of a number of virulence factors in Listeria monocytogenes. When the prfA gene was used as a DNA probe, we found it to be extremely specific for the pathogenic species L. monocytogenes. No reaction was obtained with strains of all other species of this genus. By using this information, an oligonucleotide primer pair was developed that specifically amplifies the prfA gene in L. monocytogenes strains of all known serotypes.

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页码：765 / 768

页数：4

相关论文

共 20 条

[1] DETECTION OF LISTERIA SPECIES AND LISTERIA-MONOCYTOGENES USING POLYMERASE CHAIN-REACTION [J].

BORDER, PM ;

HOWARD, JJ ;

PLASTOW, GS ;

SIGGENS, KW .

LETTERS IN APPLIED MICROBIOLOGY, 1990, 11 (03) :158-162

[2]

CHAKRABORTY T, 1988, CURR TOP MICROBIOL, V138, P41

[3] COORDINATE REGULATION OF VIRULENCE GENES IN LISTERIA-MONOCYTOGENES REQUIRES THE PRODUCT OF THE PRFA GENE [J].

CHAKRABORTY, T ;

LEIMEISTERWACHTER, M ;

DOMANN, E ;

HARTL, M ;

GOEBEL, W ;

NICHTERLEIN, T ;

NOTERMANS, S .

JOURNAL OF BACTERIOLOGY, 1992, 174 (02) :568-574

[4] A SELECTIVE DIFFERENTIAL MEDIUM FOR THE ISOLATION OF LISTERIA-MONOCYTOGENES [J].

CURTIS, GDW ;

MITCHELL, RG ;

KING, AF ;

GRIFFIN, EJ .

LETTERS IN APPLIED MICROBIOLOGY, 1989, 8 (03) :95-98

[5] DETECTION OF HEMOLYTIC LISTERIA-MONOCYTOGENES BY USING DNA COLONY HYBRIDIZATION [J].

DATTA, AR ;

WENTZ, BA ;

HILL, WE .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (09) :2256-2259

[6] MOLECULAR-CLONING, SEQUENCING, AND IDENTIFICATION OF A METALLOPROTEASE GENE FROM LISTERIA-MONOCYTOGENES THAT IS SPECIES-SPECIFIC AND PHYSICALLY LINKED TO THE LISTERIOLYSIN GENE [J].

DOMANN, E ;

LEIMEISTERWACHTER, M ;

GOEBEL, W ;

CHAKRABORTY, T .

INFECTION AND IMMUNITY, 1991, 59 (01) :65-72

[7] PASTEURIZED MILK AS A VEHICLE OF INFECTION IN AN OUTBREAK OF LISTERIOSIS [J].

FLEMING, DW ;

COCHI, SL ;

MACDONALD, KL ;

BRONDUM, J ;

HAYES, PS ;

PLIKAYTIS, BD ;

HOLMES, MB ;

AUDURIER, A ;

BROOME, CV ;

REINGOLD, AL .

NEW ENGLAND JOURNAL OF MEDICINE, 1985, 312 (07) :404-407

[8] DETECTION AND IDENTIFICATION OF LISTERIA-MONOCYTOGENES IN COOKED SAUSAGE PRODUCTS AND IN MILK BY INVITRO AMPLIFICATION OF HEMOLYSIN GENE FRAGMENTS [J].

FURRER, B ;

CANDRIAN, U ;

HOEFELEIN, C ;

LUETHY, J .

JOURNAL OF APPLIED BACTERIOLOGY, 1991, 70 (05) :372-379

[9] LISTERIOSIS [J].

GELLIN, BG ;

BROOME, CV .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1989, 261 (09) :1313-1320

[10] IDENTIFICATION OF A GENE THAT POSITIVELY REGULATES EXPRESSION OF LISTERIOLYSIN, THE MAJOR VIRULENCE FACTOR OF LISTERIA-MONOCYTOGENES [J].

LEIMEISTERWACHTER, M ;

HAFFNER, C ;

DOMANN, E ;

GOEBEL, W ;

CHAKRABORTY, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (21) :8336-8340