SYNTHESES AND TESTING OF SUBSTRATES AND MECHANISM-BASED INACTIVATORS FOR XYLANASES

被引：46

作者：

ZISER, L ^{[1
]}

SETYAWATI, I ^{[1
]}

WITHERS, SG ^{[1
]}

机构：

[1] UNIV BRITISH COLUMBIA, DEPT CHEM, VANCOUVER, BC V6T 1Z1, CANADA

来源：

CARBOHYDRATE RESEARCH | 1995年 / 274卷

关键词：

ARYL BETA-XYLOBIOSIDES; 2-DEOXY-2-HALO-BETA-D-XYLOPYRANOSIDE; 2-DEOXY-2-HALO-BETA-XYLOBIOSIDES; SUBSTRATE; INACTIVATOR; GLYCOSIDASE INHIBITORS; ENDO-XYLANASE [BETA-(1-]4)-XYLAN XYLANOHYDROLASE; EC; 3.2.1.8;

D O I：

10.1016/0008-6215(95)00080-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The syntheses of the 2,5- and 3,4-dinitrophenyl beta-xylobiosides by two separate routes are described, as well as the syntheses of the 2,4-dinitrophenyl beta-glycosides of 2-chloro-2-deoxyxylobiose and 2-deoxy-2-fluoro-xylobiose. Both the 3,4- and 2,5-dinitrophenyl beta-xylobiosides proved to be good substrates for the Bacillus subtilis xylanase, with k(cat)/K-m values of 1.0 and 34.4 mM(-1) s(-1), respectively. Excellent time-dependent inactivation of the exoxylanase/glucanase from Cellulomonas fimi was provided by 2,4-dinitrophenyl 2-deoxy-2-fluoro-beta-xylobioside,according to inactivation parameters of k(i) = 0.057 min(-1) and K-i = 0.0035 mM.

引用

页码：137 / 153

页数：17

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