DETERMINATION OF SALIVARY CORTISOL BY ELISA AND ITS APPLICATION TO THE ASSESSMENT OF THE CIRCADIAN-RHYTHM IN CHILDREN

被引:80
作者
SHIMADA, M
TAKAHASHI, K
OHKAWA, T
SEGAWA, M
HIGURASHI, M
机构
[1] UNIV TOKYO,FAC MED,DEPT MATERNAL & CHILD HLTH,TOKYO 113,JAPAN
[2] NATL CTR NEUROL & PSYCHIAT,MUSASHI HOSP,TOKYO,JAPAN
[3] TEIKYO UNIV,SCH MED,DEPT OBSTET & GYNECOL,TOKYO 173,JAPAN
[4] SEGAWA CLIN NEUROL CHILDREN,TOKYO,JAPAN
关键词
SALIVA; CORTISOL; ELISA; CIRCADIAN RHYTHM; CHILD;
D O I
10.1159/000184628
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In 35 young children, circadian rhythms of salivary cortisol levels were determined by ELISA using a commercially available kit with a minor modification. The concentration of labeled cortisol in the serum kit was reduced in order to measure cortisol in 10 mu l of saliva. Intra- and interassay coefficients of variation for salivary cortisol ranged from 2.4 to 9.9 and 3.2 to 8.9%, respectively. Recovery of salivary cortisol was 82.9-107.0%. There was a highly significant correlation between cortisol levels in saliva and serum in adults (r = 0.857). Salivary cortisol levels ranged from 0.01 to 2.252 mu g/100 ml and showed significant diurnal variation in the children. Our ELISA is a precise, simple, noninvasive and useful method for clinical practice and study in infants and children.
引用
收藏
页码:213 / 217
页数:5
相关论文
共 14 条
[1]   SALIVARY CORTISOL DETERMINATION - ADAPTATION OF A COMMERCIAL SERUM CORTISOL KIT [J].
ALANSARI, AAK ;
PERRY, LA ;
SMITH, DS ;
LANDON, J .
ANNALS OF CLINICAL BIOCHEMISTRY, 1982, 19 (MAY) :163-166
[2]  
ARNSTADT KI, 1981, J CLIN CHEM CLIN BIO, V19, P602
[3]   DIRECT RADIOIMMUNOASSAY OF CORTISOL IN SALIVA AND ITS APPLICATION TO THE DEXAMETHASONE SUPPRESSION TEST IN AFFECTIVE-DISORDERS [J].
HANADA, K ;
YAMADA, N ;
SHIMODA, K ;
TAKAHASHI, K ;
TAKAHASHI, S .
PSYCHONEUROENDOCRINOLOGY, 1985, 10 (02) :193-201
[4]  
HIRAMATSU R, 1981, CLIN CHIM ACTA, V117, P239, DOI 10.1016/0009-8981(81)90043-7
[5]  
KAWAGOE S, 1978, Folia Endocrinologica Japonica, V54, P1
[6]   VISUAL PATHWAYS AND THE ENTRAINMENT OF CIRCADIAN-RHYTHMS [J].
MOORE, RY ;
CARD, JP .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1985, 453 :123-133
[7]   SALIVARY TESTOSTERONE CONCENTRATIONS IN PREPUBERTAL AND PUBERTAL MALES - COMPARISON WITH TOTAL AND FREE PLASMA TESTOSTERONE [J].
OHZEKI, T ;
MANELLA, B ;
GUBELINDECAMPO, C ;
ZACHMANN, M .
HORMONE RESEARCH, 1991, 36 (5-6) :235-237
[8]  
ONISHI S, 1983, PEDIATRICS, V72, P399
[9]  
RIADFAHMY D, 1981, J CLIN CHEM CLIN BIO, V19, P812
[10]   DEVELOPMENT OF THE SLEEP AND WAKEFULNESS RHYTHM IN PRETERM INFANTS DISCHARGED FROM A NEONATAL CARE UNIT [J].
SHIMADA, M ;
SEGAWA, M ;
HIGURASHI, M ;
AKAMATSU, H .
PEDIATRIC RESEARCH, 1993, 33 (02) :159-163