MONOCLONAL-ANTIBODY AGAINST HUMAN GLIOBLASTOMA-MULTIFORME (U-87MG) IMMUNOPRECIPITATES A PROTEIN OF MOLECULAR-MASS 38-KDA AND INHIBITS TUMOR-GROWTH IN NUDE-MICE

被引：4

作者：

DASTIDAR, SG ^{[1
]}

SHARMA, SK ^{[1
]}

机构：

[1] ALL INDIA INST MED SCI,DEPT BIOCHEM,NEW DELHI 110029,INDIA

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1995年 / 56卷 / 01期

关键词：

GLIOBLASTOMA MULTIFORME; MONOCLONAL ANTIBODY; BRAIN TUMOR; U-87MG;

D O I：

10.1016/0165-5728(94)00136-C

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A monoclonal antibody 6DS(1) against a human glioblastoma multiforme cell line U-87MG recognizes a tumor-specific, cell surface antigen of human glioblastoma cell lines. Partial cross-reactivity is observed with two human neuroblastoma cell lines, SK-N-SH and SK-N-MC, with little or no reactivity towards a rat glioma cell line C6 or normal human adult and fetal brain tissues. The antibody recognizes an antigen of molecular mass 38 kDa as inferred from Western blot analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the immunoprecipitate. The monoclonal antibody 6DS(1) inhibits both the attachment to substratum and growth of U-87MG cells. It strongly cross-reacts with xenotransplants of U-87MG cells and inhibits tumorigenesis (subcutaneous implants of U-87MG cells) in nude mice.

引用

页码：91 / 98

页数：8

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