GERANYLGERANYL PYROPHOSPHATE AS CONDENSING UNIT FOR ENZYMATIC SYNTHESIS OF CAROTENES

被引:27
作者
LEE, TC
CHICHEST.CO
机构
[1] Department of Food Science and Technology, University of California, Davis
关键词
D O I
10.1016/S0031-9422(00)85407-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A cell-free enzyme system capable of converting 4,8,12,16-trans-14C-geranylgeranyl pyrophosphate into phytoene, phytofluene, ζ-carotene, neurosporene, and lycopene was obtained from a Phycomyces blakesleeanus R1 mutant. The conditions of incubation and cofactor requirements for the conversion were defined. The incorporation of radioactive substrate into carotenes was maximal at pH 7·8 with Tris-HCl buffer and in the presence of a sulfhydryl reagent (dithiothreitol) at 25° for 24 hr. Geranylgeranyl pyrophosphate was a much more effective precursor than mevalonic acid in this system and it was demonstrated that geranylgeranyl pyrophosphate is the condensing unit for carotene biosynthesis. © 1969.
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页码:603 / &
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