ENTRAPMENT OF PLASMID DNA BY LIPOSOMES AND THEIR INTERACTIONS WITH PLANT-PROTOPLASTS

被引：71

作者：

LURQUIN, PF

机构：

[1] Radiobiology Department, Centre d'Etude de l'Energie Nucléaire, C.E.N./S.C.K.

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 6卷 / 12期

关键词：

D O I：

10.1093/nar/6.12.3773

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lecithin and lecithin/cholesterol liposomes formed in aqueous solu tions of DNA entrap covalently closed circular, open circular and linear DNA molecules of size up to at least 13 kilobases. The sequestered DNA molecules are efficiently protected against exogenous deoxyribonuclease action although nicking and linearization of circular DNA can be observed. The size of these liposomes ranges from approximately 0.5 to 7.5 μ with an average of 2.5-4 μ. DNA filled liposomes strongly interact with plant protoplasts under conditions inducing protoplast fusion. Results suggest that sequestered plasmid DNA can be transferred to protoplsst nuclei. © 1979 Information Retrieval Limited.

引用

页码：3773 / 3784

页数：12

共 34 条

[1] USE OF AN ABRASIVE IN ISOLATION OF COWPEA LEAF PROTOPLASTS WHICH SUPPORT MULTIPLICATION OF COWPEA MOSAIC-VIRUS [J].