URIC-ACID AS ELECTRON-ACCEPTOR OF LIVER XANTHINE DEHYDROGENASE

被引：5

作者：

CANELA, EI

BOZAL, J

机构：

[1] Departamento de Bioquimica, Facultad de Quimica, Universidad de Barcelona

来源：

JOURNAL OF MOLECULAR CATALYSIS | 1979年 / 6卷 / 02期

关键词：

D O I：

10.1016/0304-5102(79)80022-X

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The stoichiometry of the hypoxanthine + uric acid → 2 xanthine reaction, catalysed by chicken liver xanthine dehydrogenase (xanthine: NAD+-oxidoreductase (E.C. 1.2.1.37)), has been determined by u.v. spectrophotometry. The system is adapted to a ping-pong type mechanism; in the proposed reaction sequence, the hypoxanthine is oxidised to xanthine and the reduced form of the enzyme is oxidised by the uric acid, whereby a second xanthine molecule is formed. The reaction product, xanthine, is a non-competitive inhibitor of the enzyme relative to both substrates. Hypoxanthine and excess uric acid are competitive, linear inhibitors of the system. © 1979.

引用

页码：153 / 162

页数：10

共 11 条

[1] BRAY RC, 1975, ENZYMES, V12, P365
[2] KINETICS OF ENZYME-CATALYZED REACTIONS WITH 2 OR MORE SUBSTRATES OR PRODUCTS .2. INHIBITION - NOMENCLATURE AND THEORY
CLELAND, WW
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1963, 67 (02) : 173 - &
[3] GRAPHICAL DETERMINATION OF THE DISSOCIATION CONSTANTS FOR 2-SUBSTRATE ENZYME SYSTEMS
FLORINI, JR
VESTLING, CS
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1957, 25 (03) : 575 - 578
[4] Studies on reversible dehydrogenase systems. II. The reversibility of the xanthine oxidase system.
Green, DE
[J]. BIOCHEMICAL JOURNAL, 1934, 28 : 1550 - 1560
[5] GUBERT S, 1972, REV ESP FISIOL, V28, P213
[6] A SCHEMATIC METHOD OF DERIVING THE RATE LAWS FOR ENZYME-CATALYZED REACTIONS
KING, EL
ALTMAN, C
[J]. JOURNAL OF PHYSICAL CHEMISTRY, 1956, 60 (10) : 1375 - 1378
[7] LOPEZSOLANAS V, 1975, REV ESP FISIOL, V31, P255
[8] PLOWMAN KM, 1972, ENZYME KINETICS, P66
[9] PRIEST DE, 1968, EUR J BIOCH, V10, P439
[10] NONLINEAR KINETICS OF XANTHINE OXIDASE REACTION CATALYZED BY CHICKEN LIVER XANTHINE DEHYDROGENASE
PRIEST, DG
FISHER, JR
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1971, 146 (02) : 391 - &

← 1 2 →