DIRECT ELECTRON-TRANSFER BETWEEN CARBON ELECTRODES, IMMOBILIZED MEDIATOR AND AN IMMOBILIZED VIOLOGEN-ACCEPTING PYRIDINE-NUCLEOTIDE OXIDOREDUCTASE

The electron transfer between electrodes and the redox‐active prosthetic groups of oxidoreductases is usually too slow to be measurable, unless a mediator is used. Now, a mediator and the enzyme have been covalently immobilized on a functionalized carbon electrode. The electrons can thus flow from the cathode to the oxidoreductase via a “molecular wire”. The use of carbon felt having a larger inner surface area as electrode affords current densities at the lower end of the range used in industrial processes. Copyright © 1990 by VCH Verlagsgesellschaft mbH, Germany