LONG-TERM CULTURED NEURONS FROM RAT SUPRACHIASMATIC NUCLEUS RETAIN THE CAPACITY FOR CIRCADIAN OSCILLATION OF VASOPRESSIN RELEASE

被引:104
作者
MURAKAMI, N [1 ]
TAKAMURE, M [1 ]
TAKAHASHI, K [1 ]
UTUNOMIYA, K [1 ]
KURODA, H [1 ]
ETOH, T [1 ]
机构
[1] NATL CTR NERVOUS MENTAL & MUSCULAR DISORDERS, KODAIRA, TOKYO 187, JAPAN
关键词
CIRCADIAN RHYTHM; SUPRACHIASMATIC NUCLEUS; VASOPRESSIN; CELL CULTURE;
D O I
10.1016/0006-8993(91)91312-O
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We have developed a dissociated suprachiasmatic nucleus (SCN) cell culture system in order to begin a cellular analysis of the mammalian circadian oscillator. Monolayer-cultured neurons prepared from the SCN regions of 1-day-old rats were maintained in serum-free culture medium for 3-4 weeks and then perfused. In 12 out of 40 wells, a circadian oscillation of vasopressin release persisted for at least 4-5 cycles until the end of the perfusion period with no damping of the amplitude. This finding suggests that cultured SCN neurons retain the capacity for circadian oscillation for a long time, and should provide a useful model for the analysis of a mammalian circadian system at the cellular level.
引用
收藏
页码:347 / 350
页数:4
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