CLONING OF A SEQUENCE OF AQUASPIRILLUM-MAGNETOTACTICUM THAT COMPLEMENTS THE AROD GENE OF ESCHERICHIA-COLI

被引：9

作者：

BERSON, AE ^{[1
]}

HUDSON, DV ^{[1
]}

WALEH, NS ^{[1
]}

机构：

[1] SRI INT,DEPT MOLEC & CELL BIOL,333 RAVENSWOOD AVE,MENLO PK,CA 94025

来源：

MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 09期

关键词：

D O I：

10.1111/j.1365-2958.1991.tb02156.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 2 kb DNA fragment isolated from a cosmid library of Aquaspirillum magnetotacticum strain MS-1 complements the aromatic-metabolite requirements and iron-uptake deficiencies of Escherichia coli and Salmonella typhimurium strains that lack a functional aroD (biosynthetic dehydrodquinase) sequence. All recombinant cosmids selected for their aroD complementation property carry this sequence. No DNA sequence homology has, however, been detected by Southern hybridization between the cloned fragment and the aroD gene of E. coli or the qa2 (catabolic dehydroquinase) gene of Neurospora crassa.

引用

页码：2261 / 2264

页数：4

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