MASS-SPECTROMETRY SOFTWARE FOR BIOCHEMICAL-ANALYSIS IN ELECTROSPRAY AND FAST-ATOM-BOMBARDMENT MODES

被引:11
作者
WATKINS, PJF [1 ]
JARDINE, I [1 ]
ZHOU, JXG [1 ]
机构
[1] FINNIGAN MAT CORP,SAN JOSE,CA 95134
关键词
D O I
10.1042/bst0190957
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
New mass spectrometry techniques, such as electrospray ionization (ESI), allow the study of large biomolecules and peptide mixtures. The data produced are complex and interpretation can be a long and tedious process. A new suite of data-processing software is described which allows many of these operations to be carried out in a rapid, automated way. Software is described for the deconvolution of the spectra of multiply charged ions, for both pure compounds and mixtures. The rapid peptide mapping of protein digests from h.p.l.c.-m.s. data and peptide sequence confirmation from multiple-stage (MS)-m.s. data using tandem quadrupole m.s. are also described. In addition preliminary results are presented on first principle sequencing of unknown peptides from MS-m.s. experiments.
引用
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页码:957 / 962
页数:6
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