PURIFICATION AND PROPERTIES OF COLLAGENASE FROM CYTOPHAGA SP L43-1 STRAIN

被引：30

作者：

SASAGAWA, Y ^{[1
]}

KAMIO, Y ^{[1
]}

MATSUBARA, Y ^{[1
]}

SUZUKI, K ^{[1
]}

KOJIMA, H ^{[1
]}

IZAKI, K ^{[1
]}

机构：

[1] NIPPI INC, RES INST, ADACHI KU, TOKYO 120, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 11期

关键词：

D O I：

10.1271/bbb.57.1894

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A collagenolytic bacterial strain was isolated from soil and was identified as Cytophaga sp. It produced several kinds of collagenase and protease. From the supernatant of a culture, a collagenase was purified as a single protein band upon SDS-PAGE and its molecular mass was estimated to be 120 kDa. Collagen and gelatin were good substrates for this enzyme. Beta-casein was cleaved by this enzyme at several sites.

引用

页码：1894 / 1898

页数：5

共 21 条

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