EXTENDING THE RANGE OF APPLICATION OF THE BIURET REACTION - QUANTITATIVE-DETERMINATION OF INSOLUBLE PROTEINS

被引：9

作者：

GOSHEV, I

NEDKOV, P

机构：

[1] Institute of Organic Chemistry, Bulgarian Academy of Sciences, Sofia

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90736-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for quantitative determination of insoluble proteins by means of the biuret reaction is proposed. The sample is oxidized with excess H2O2, dried, and subsequently dissolved in 0.05% sodium dodecyl sulfate-1 n NaOH at 100°C. The clear solution obtained is treated with biuret reagent and the absorbance of the chromogen is measured at 35-40°C. The method is applied successfully to collagen from bovine Achilles tendon, keratin of sheep's wool, and defatted sheepskin. Biuret chromogen absorbance of H2O2-oxidized human serum albumin is 10 ± 0.5% lower than that obtained with the native protein. © 1979.

引用

页码：340 / 343

页数：4

共 11 条

[1]

Devenyi T, 1974, AMINO ACIDS PEPTIDES

[2] APPLICATION OF THE WEICHSELBAUM BIURET REAGENT TO THE DETERMINATION OF SPINAL FLUID PROTEIN [J].