DIURNAL-VARIATIONS OF RAT-LIVER ENZYMES CATALYZING CHOLESTEROL ESTER HYDROLYSIS

被引：14

作者：

MARTINEZ, MJ ^{[1
]}

RUIZ, JI ^{[1
]}

LACORT, M ^{[1
]}

OCHOA, B ^{[1
]}

机构：

[1] UNIV BASQUE COUNTRY,SCH MED,DEPT PHYSIOL,AP 699,E-48080 BILBAO,SPAIN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1085卷 / 01期

关键词：

CHOLESTEROL ESTERASE; DIURNAL VARIATION; (RAT LIVER);

D O I：

10.1016/0005-2760(91)90237-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cholesterol ester hydrolase activity was determined at 3 h time intervals over 24 h in lysosomes, cytosol and microsomes from ad libitum-fed and 24 h food-deprived female rat liver. Diurnal rhythms were identified for the acid and neutral esterases, which were strikingly changed by fasting. In fed animals, lysosomal esterase specific activity exhibited a peak at noon and a sustained medium rate at early darkness, whereas total esterase was maximal at midnight. The circadian patterns of the cytosolic and the microsomal esterases paralelled each other, though the amplitude of rhythms differed, showing higher activities around midnight. After fasting, cholesterol esterase activity from all cell fractions reached a maximum near dark onset. These results are the first to indicate that cholesteryl ester hydrolysis may play a role in generating the diurnal rhythm of hepatic cholesterol.

引用

页码：106 / 111

页数：6

共 35 条

[1] SUBMICROSOMAL LOCALIZATION OF ACYL-COENZYME A-CHOLESTEROL ACYLTRANSFERASE AND ITS SUBSTRATE, AND OF CHOLESTERYL ESTERS IN RAT-LIVER [J].