MEASUREMENT OF CELL-PROLIFERATION BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) USING A MONOCLONAL-ANTIBODY TO BROMODEOXYURIDINE

被引：32

作者：

PERROS, P ^{[1
]}

WEIGHTMAN, DR ^{[1
]}

机构：

[1] ROYAL VICTORIA INFIRM,SUPRAREG ASSAY SERV LABS,NEWCASTLE TYNE NE1 4LP,TYNE & WEAR,ENGLAND

来源：

CELL PROLIFERATION | 1991年 / 24卷 / 05期

关键词：

D O I：

10.1111/j.1365-2184.1991.tb01179.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

An ELISA was developed and optimized to measure cell proliferation using a monoclonal antibody to bromodeoxyuridine (BrdUrd). Incorporation of BrdUrd into myoblast monolayers, measured as the optical density at 492 nm, increased in response to fetal calf serum, IGF-I and EGF, the ELISA data correlated closely with data obtained by BrdUrd immunocytochemistry (r = 0.984), cell counting (r = 0.972) and tritiated thymidine uptake by liquid scintillation counting (r = 0.990). The BrdUrd ELISA is a useful alternative to measurement of tritiated thymidine uptake by scintillation counting, and has the added advantages of dispensing with the use of radioactivity and of being less labour intensive.

引用

页码：517 / 523

页数：7

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