THE EXPRESSION PATTERN OF THE CHICK HOMEOBOX GENE GMHOX SUGGESTS A ROLE IN PATTERNING OF THE LIMBS AND FACE AND IN COMPARTMENTALIZATION OF SOMITES

被引:115
作者
KURATANI, S [1 ]
MARTIN, JF [1 ]
WAWERSIK, S [1 ]
LILLY, B [1 ]
EICHELE, G [1 ]
OLSON, EN [1 ]
机构
[1] UNIV TEXAS,MD ANDERSON CANC CTR,DEPT BIOCHEM & MOLEC BIOL,HOUSTON,TX 77030
关键词
D O I
10.1006/dbio.1994.1037
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
MHox is a homeodomain protein that binds an essential element in the core of the muscle creatine kinase enhancer. In the mouse embryo, MHox expression is restricted to mesenchymal cells; in adult mice the gene is highly expressed in skeletal and cardiac muscle. To further define the functions of MHox during embryogenesis, we have cloned its chicken homolog, termed gMHox, and analyzed its properties and detailed expression patterns. Our studies show that the amino acid sequence and DNA-binding properties of the avian and murine gene products are very similar. Furthermore, the sites of expression are alike with high levels of expression in the splanchnic mesoderm, in the somatic mesoderm, in the limb bud mesoderm, in the dermatome and in the dermis, and in the ectomesenchyme of the face. gMHox became downregulated as chondrogenesis proceeded, whereas its expression was maintained in perichondrium and undifferentiated mesenchymal cells beneath the surface ectoderm. Such a pattern of expression suggests that gMHox may participate in maintenance of mesenchymal cell lineages derived from both mesoderm and the neural crest and in patterning of the limbs and the face. Removal of the surface ectoderm overlying the somites has no visible effect on the architecture of somites but results in the failure of gMHox to be expressed in the underlying dermatome, suggesting that regulation of gMHox expression in these cells is dependent on cues emanating from the surface ectoderm. © 1994 by Academic Press, Inc.
引用
收藏
页码:357 / 369
页数:13
相关论文
共 40 条
[1]  
CHEVALLIER A, 1977, J EMBRYOL EXP MORPH, V41, P245
[2]  
CHEVALLIER A, 1977, J EMBRYOL EXP MORPH, V43, P263
[3]   ORIGIN OF FLIGHT MUSCULATURE - EXPERIMENTAL STUDIES WITH QUAIL AND CHICK-EMBRYOS [J].
CHRIST, B ;
JACOB, HJ ;
JACOB, M .
EXPERIENTIA, 1974, 30 (12) :1446-1449
[4]   EXPERIMENTAL STUDIES ON DEVELOPMENT OF CHESTWALL IN CHICK-EMBRYOS [J].
CHRIST, B ;
JACOB, HJ ;
JACOB, M .
EXPERIENTIA, 1974, 30 (12) :1449-1451
[5]  
COULY GF, 1993, DEVELOPMENT, V117, P409
[6]  
CSERJESI P, 1992, DEVELOPMENT, V115, P1087
[7]   POSITION-DEPENDENT EXPRESSION OF 2 RELATED HOMEOBOX GENES IN DEVELOPING VERTEBRATE LIMBS [J].
DAVIDSON, DR ;
CRAWLEY, A ;
HILL, RE ;
TICKLE, C .
NATURE, 1991, 352 (6334) :429-431
[8]   THE HOMEOBOX GENE-S8 - MESODERM-SPECIFIC EXPRESSION IN PRESOMITE EMBRYOS AND IN CELLS CULTURED INVITRO AND MODULATION IN DIFFERENTIATING PLURIPOTENT CELLS [J].
DEJONG, R ;
MEIJLINK, F .
DEVELOPMENTAL BIOLOGY, 1993, 157 (01) :133-146
[9]   A GENE WITH HOMOLOGY TO THE MYC SIMILARITY REGION OF MYOD1 IS EXPRESSED DURING MYOGENESIS AND IS SUFFICIENT TO ACTIVATE THE MUSCLE DIFFERENTIATION PROGRAM [J].
EDMONDSON, DG ;
OLSON, EN .
GENES & DEVELOPMENT, 1989, 3 (05) :628-640
[10]   A NEW MYOCYTE-SPECIFIC ENHANCER-BINDING FACTOR THAT RECOGNIZES A CONSERVED ELEMENT ASSOCIATED WITH MULTIPLE MUSCLE-SPECIFIC GENES [J].
GOSSETT, LA ;
KELVIN, DJ ;
STERNBERG, EA ;
OLSON, EN .
MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (11) :5022-5033