XYLOSE-SPECIFIC ANTIBODIES AS MARKERS OF SUBCOMPARTMENTATION OF TERMINAL GLYCOSYLATION IN THE GOLGI-APPARATUS OF SYCAMORE CELLS

被引：35

作者：

LAINE, AC ^{[1
]}

GOMORD, V ^{[1
]}

FAYE, L ^{[1
]}

机构：

[1] UNIV ROUEN HAUTE NORMANDIE,FAC SCI,TRANSPORTS INTRACELLULAIRES,CNRS,UA 203,F-76134 MT ST AIGNAN,FRANCE

来源：

FEBS LETTERS | 1991年 / 295卷 / 1-3期

关键词：

XYLOSYLTRANSFERASE IMMUNOLOCALIZATION; PLANT GLYCOPROTEIN BIOSYNTHESIS; PLANT GLYCOSYLTRANSFERASE; PLANT GOLGI APPARATUS;

D O I：

10.1016/0014-5793(91)81413-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Antibodies specific for xylose-containing plant complex N-linked glycans are used for indirect immunolocalization of xylosyltransferase in sycamore cells. The use of high pressure freezing and freeze substitution for sample preparation resulted in very good morphological preservation of the different Golgi cisternae. Xylosyltransferase shows a diffuse distribution all over the Golgi stacks and xylosylation appears to be an early processing event that is initiated in the cis Golgi compartment.

引用

页码：179 / 184

页数：6

共 30 条

[1] GOLGI-SPECIFIC LOCALIZATION OF TRANSGLYCOSYLASES ENGAGED IN GLYCOPROTEIN-BIOSYNTHESIS IN SUSPENSION-CULTURED CELLS OF SYCAMORE (ACER-PSEUDOPLATANUS L) [J].

ALI, MS ;

MITSUI, T ;

AKAZAWA, T .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1986, 251 (02) :421-431

[2]

CHRISPEELS MJ, 1991, ANNU REV PLANT PHYS, V42, P21, DOI 10.1146/annurev.pp.42.060191.000321

[3]

CRAIG S, 1988, EUR J CELL BIOL, V46, P80

[4] REGENERATION OF FERTILE PLANTS FROM PROTOPLASTS OF DIFFERENT ARABIDOPSIS-THALIANA GENOTYPES [J].

DAMM, B ;

WILLMITZER, L .

MOLECULAR & GENERAL GENETICS, 1988, 213 (01) :15-20

[5] THE ROLE OF HIGH-MANNOSE AND COMPLEX ASPARAGINE-LINKED GLYCANS IN THE SECRETION AND STABILITY OF GLYCOPROTEINS [J].

DRIOUICH, A ;

GONNET, P ;

MAKKIE, M ;

LAINE, AC ;

FAYE, L .

PLANTA, 1989, 180 (01) :96-104

[6] COMPARTMENTAL ORGANIZATION OF THE GOLGI STACK [J].

DUNPHY, WG ;

ROTHMAN, JE .

CELL, 1985, 42 (01) :13-21

[7]

ELBEIN AD, 1987, ANNU REV BIOCHEM, V56, P497, DOI 10.1146/annurev.biochem.56.1.497

[8] COMMON ANTIGENIC DETERMINANTS IN THE GLYCOPROTEINS OF PLANTS, MOLLUSKS AND INSECTS [J].

FAYE, L ;

CHRISPEELS, MJ .

GLYCOCONJUGATE JOURNAL, 1988, 5 (03) :245-256

[9] TRANSPORT AND POSTTRANSLATIONAL PROCESSING OF THE VACUOLAR ENZYME ALPHA-MANNOSIDASE IN JACK-BEAN COTYLEDONS [J].

FAYE, L ;

GREENWOOD, JS ;

HERMAN, EM ;

STURM, A ;

CHRISPEELS, MJ .

PLANTA, 1988, 174 (02) :271-282

[10] CHARACTERIZATION OF N-LINKED OLIGOSACCHARIDES BY AFFINOBLOTTING WITH CONCANAVALIN-A PEROXIDASE AND TREATMENT OF THE BLOTS WITH GLYCOSIDASES [J].

FAYE, L ;

CHRISPEELS, MJ .

ANALYTICAL BIOCHEMISTRY, 1985, 149 (01) :218-224

← 1 2 3 →